来自中国的[具体物种名称未给出]线粒体全基因组测序与分析及其系统发育分析。
Sequencing and analysis of the complete mitochondrial genome of from China and its phylogenetic analysis.
作者信息
Cai He, Wang Qing-Qing, Zhao Xin-Xu, Yao Qian-Qian, Wu Ning, Zhang Jun-Sheng, Liu Zhu
机构信息
College of Life Science and Technology, Mudanjiang Normal University, Mudanjiang, P.R. China.
Ningan Jiangnan School, Ningan, P.R. China.
出版信息
Mitochondrial DNA B Resour. 2021 May 12;6(5):1617-1620. doi: 10.1080/23802359.2021.1926353.
The complete mitogenome sequence of was determined using long PCR. The genome was 16,238 bp in length and contained 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, 1 origin of L strand replication and 1 control region. The overall base composition of the heavy strand is A (33.7%), C (24.8%), T (29.1%) and G (12.4%). The base compositions present clearly the A-T skew, which is most obviously in the control region and protein-coding genes. Mitochondrial genome analyses based on MP, ML, NJ and Bayesian analyses yielded identical phylogenetic trees. This study verifies the evolutionary status of in Muridae at the molecular level. The mitochondrial genome would be a significant supplement for the genetic background.
使用长PCR技术测定了[物种名称]的完整线粒体基因组序列。该基因组长度为16,238 bp，包含13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因、1个轻链复制起点和1个控制区。重链的总体碱基组成为A（33.7%）、C（24.8%）、T（29.1%）和G（12.4%）。碱基组成明显呈现A-T偏斜，在控制区和蛋白质编码基因中最为明显。基于最大简约法（MP）、最大似然法（ML）、邻接法（NJ）和贝叶斯分析的线粒体基因组分析产生了相同的系统发育树。本研究在分子水平上验证了[物种名称]在鼠科中的进化地位。线粒体基因组将为[物种名称]的遗传背景提供重要补充。
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