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传统中药(豆科)叶绿体全基因组的特征分析

Characterization of the complete chloroplast genome of (Leguminosae), a traditional Chinese medicine.

作者信息

Jia Guolun, Li Peng, Zhu Qiang, Peng Li

机构信息

Northwest University, Xi'an, China.

The State Key Laboratory of Seeding Bioengineering, Ningxia Forestry Intitute, Yinchuan, China.

出版信息

Mitochondrial DNA B Resour. 2019 Sep 18;4(2):3040-3041. doi: 10.1080/23802359.2019.1666050.

DOI:10.1080/23802359.2019.1666050
PMID:33365846
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7706715/
Abstract

is a tradational Chinese medicine of Leguminosae, which contains many chemicals, such as glycyrrhizin, liquiritin glycyrol, melatonin (-acetyl-5-methoxy tryptamine), glycyrrhizol A, glycyrrhizol B and four known isoflavonoids (5-O-methylglycryol, isoglycyrol, 6,8-diisoprenyl-5,7,4'-trihydroxy isoflavone, gancaonin). Illumina paired-end reads data was used to assemble the complete chloroplast (cp) genome. About 15,445,866 raw Paired-End Reads and the length distribution in 127,702 bp (GC content accounts for 34.3%). Eight PCG genes and six tRNA genes possess a single intron, while ycf3 has a couple of introns. Based on the concatenated coding sequences of cp PCGs, the phylogenetic analysis showed that and () are closely related to each other within the family Leguminosae.

摘要

是豆科的一种传统中药,它含有许多化学成分,如甘草甜素、甘草苷、甘草醇、褪黑素(N-乙酰-5-甲氧基色胺)、甘草根醇A、甘草根醇B以及四种已知的异黄酮(5-O-甲基甘草醇、异甘草醇、6,8-二异戊烯基-5,7,4'-三羟基异黄酮、甘草黄酮)。利用Illumina双末端读段数据组装完整的叶绿体(cp)基因组。约15445866条原始双末端读段,长度分布为127702bp(GC含量占34.3%)。八个PCG基因和六个tRNA基因有一个内含子,而ycf3有两个内含子。基于cp PCGs的串联编码序列,系统发育分析表明,在豆科植物中,[此处原文缺失相关物种名称]和[此处原文缺失相关物种名称]([此处原文缺失相关物种名称])彼此密切相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8de/7706715/79ef5859b2a1/TMDN_A_1666050_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8de/7706715/79ef5859b2a1/TMDN_A_1666050_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8de/7706715/79ef5859b2a1/TMDN_A_1666050_F0001_B.jpg

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本文引用的文献

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J Pharmacol Sci. 2018 Aug;137(4):324-332. doi: 10.1016/j.jphs.2018.03.006. Epub 2018 May 5.
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MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets.MEGA7:适用于更大数据集的分子进化遗传学分析版本7.0
Mol Biol Evol. 2016 Jul;33(7):1870-4. doi: 10.1093/molbev/msw054. Epub 2016 Mar 22.
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Reconstructing mitochondrial genomes directly from genomic next-generation sequencing reads--a baiting and iterative mapping approach.
直接从基因组下一代测序读段重建线粒体基因组——一种诱饵和迭代映射方法。
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OrganellarGenomeDRAW--a suite of tools for generating physical maps of plastid and mitochondrial genomes and visualizing expression data sets.细胞器基因组绘图软件--一套用于生成质体和线粒体基因组物理图谱以及可视化表达数据集的工具。
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W575-81. doi: 10.1093/nar/gkt289. Epub 2013 Apr 22.
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Gene relocations within chloroplast genomes of Jasminum and Menodora (Oleaceae) are due to multiple, overlapping inversions.素馨属和刺茉莉属(木犀科)叶绿体基因组内的基因重排是由多个重叠倒位引起的。
Mol Biol Evol. 2007 May;24(5):1161-80. doi: 10.1093/molbev/msm036. Epub 2007 Feb 28.
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J Pineal Res. 2006 Sep;41(2):108-15. doi: 10.1111/j.1600-079X.2006.00337.x.
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