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传统中药材远志变种的叶绿体全基因组特征分析

Characterization of the complete chloroplast genome of var. Bunge, a traditional Chinese medicinal plant.

作者信息

Li Peng, Yu Pei, Xia Jin

机构信息

Department of Pharmacy, School of Medicine, Xi'an International University, Xi'an, China.

出版信息

Mitochondrial DNA B Resour. 2020 Dec 24;5(4):3848-3850. doi: 10.1080/23802359.2020.1841578.

Abstract

var. Bunge. is a traditional Chinese medicinal plant of Caryophyllaceae, which contains many chemicals, such as -carboline alkaloids, sterols, cyclic peptides, phenolic acids, and flavonoids and so on, which was used as folk medicines for antifebrile to treat fever and to inhibit the cell growth, cyclooxygenase, and also can anti-inflammatory, anti-allergic, vasodilator, and other effects. Illumina paired-end reads data were used to assemble the complete chloroplast (cp) genome. 16,898,032 raw paired-end reads and the length distribution in 150,602 bp, including a large single-copy (LSC) region of 81,844 bp, a small single-copy (SSC) region of 17,022 bp and a pair of inverted repeats (IRs) of 25,868 bp. Besides, nine protein-coding genes (PCGs) genes and six tRNA genes possess a single intron, while clpP and ycf3 have a couple of introns. Based on the concatenated coding sequences of cp PCGs, the phylogenetic analysis showed that and (MK309611) are closely related to each other within the family Caryophyllaceae.

摘要

变种。 Bunge。是石竹科的一种传统中药材,含有许多化学成分,如β-咔啉生物碱、甾醇、环肽、酚酸和黄酮类等,被用作民间药物用于退热、治疗发热和抑制细胞生长、环氧化酶,还具有抗炎、抗过敏、血管舒张等作用。利用Illumina双端读段数据组装完整的叶绿体(cp)基因组。16,898,032条原始双端读段,长度分布为150,602 bp,包括一个81,844 bp的大单拷贝(LSC)区域、一个17,022 bp的小单拷贝(SSC)区域和一对25,868 bp的反向重复序列(IRs)。此外,9个蛋白质编码基因(PCGs)和6个tRNA基因有一个内含子,而clpP和ycf3有两个内含子。基于cp PCGs的串联编码序列,系统发育分析表明, 与 (MK309611)在石竹科内彼此密切相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c145/7759295/4dc64533e72c/TMDN_A_1841578_F0001_B.jpg

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