Zhao Yaling, Wang Yang, Liu Yang
Key Laboratory of Resource Biology and Biotechnology in Western China (Ministry of Education), Shaanxi Key Laboratory for Animal Conservation, College of Life Science, Northwest University, Xi'an, PR China.
College of Biology Pharmacy and Food Engineering, Shangluo University, Shangluo, PR China.
Mitochondrial DNA B Resour. 2019 Oct 4;4(2):3340-3341. doi: 10.1080/23802359.2019.1673239.
In this study, we determined the complete mitochondrial genome sequence of click beetle (Heyden, 1887) (GenBank accession no. MN306531) using next-generation sequencing (NGS) method. The mitogenome is 15,842 bp in length, consisting of 13 protein-coding genes (PCGs), two ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and one non-coding control region. The overall nucleotide composition was 41.6% A, 31.1% T, 16.9% C, and 10.4% G, with 72.7% AT, respectively. The gene arrangement is consistent with the typical insect mitochondrial genome. Phylogenetic analysis revealed that clustered into a clade with with high bootstrap support.
在本研究中,我们使用下一代测序(NGS)方法确定了叩头虫(Heyden,1887)(GenBank登录号:MN306531)的完整线粒体基因组序列。该线粒体基因组长度为15,842 bp,由13个蛋白质编码基因(PCGs)、两个核糖体RNA基因(rRNAs)、22个转运RNA基因(tRNAs)和一个非编码控制区组成。总体核苷酸组成分别为A占41.6%、T占31.1%、C占16.9%、G占10.4%,AT含量为72.7%。基因排列与典型昆虫线粒体基因组一致。系统发育分析表明,在高自展支持下,与聚为一个分支。