The mitochondrial genome analysis of a stonefly, Okamoto, 1922 (Plecoptera: Nemouridae).

To better understand the diversity and phylogeny of Nemouridae, we sequenced and annotated the complete mitochondrial genome (mitogenome) of Okamoto, 1922. The entire mitochondrial genome of was 15,728 bp. It contained 37 representative genes (22 transfer RNA genes (tRNAs), 13 protein-coding genes (PCGs), and two ribosomal RNA genes (rRNAs)) and a control region. In this genome, 23 genes were found on the heavy strand (H-strand) and 14 genes were on the light strand (L-strand). All PCGs began with the initiation codon ATN. Eleven PCGs stopped with the termination codon TAA or TAG, while and genes stopped with incomplete codon T. Bayesian's inference (BI) and maximum-likelihood (ML) methods generated the identical tree topology across the PCGR dataset (13 PCGs plus two rRNAs). The monophyly of each subfamily was well-supported. This analysis supported the clade plus as sister taxon to the clade plus .