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一种石蝇(襀翅目:石蝇科)线粒体全基因组的特征分析

Characterization of the complete mitochondrial genome of a stonefly species,  (Plecoptera: Pelidae).

作者信息

Chen Jiajia, Cao Jinjun, Li Weihai, Wang Ying

机构信息

Department of Plant Protection, Henan Institute of Science and Technology, Xinxiang, China.

出版信息

Mitochondrial DNA B Resour. 2019 Oct 9;4(2):3416-3417. doi: 10.1080/23802359.2019.1674722.

DOI:10.1080/23802359.2019.1674722
PMID:33366019
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7710318/
Abstract

We have sequenced and analyzed complete mitochondrial genome (mitogenomes) of the , the third mitochondrial genome in the genus which belongs to the family Pelidae in this paper. The mitogenome of  is circular with the length of 16,077 bp, which possessed 37 genes and a control region like other stonefly. The A + T content of the whole mitogenome was 67.1%. All PCGs were 11,217 bp in length, accounting for 65.3% of the content of A + T. The A + T content of lrRNA and srRNA were 72.3% and 66.4%. The highest A + T content was in the control region (76.2%). There are 15 gene overlaps and 9 gene intergenic spacers in this mitochondrial genome. In addition, we performed phylogenetic analysis by using the Bayesian (BI) and Maximum Likelihood (ML) methods based on the concatenated data set of PCGs from 11 species in Pelidae and two species in Styloperlidae (outgroups). The clade + was a sister group to , which is consistent with the traditional morphological classification.

摘要

我们已对[物种名称]的完整线粒体基因组(线粒体基因组)进行了测序和分析,这是本文中属于襀科的[属名]属中的第三个线粒体基因组。[物种名称]的线粒体基因组呈环状,长度为16,077 bp,与其他石蝇一样拥有37个基因和一个控制区。整个线粒体基因组的A+T含量为67.1%。所有编码蛋白质的基因(PCGs)长度为11,217 bp,占A+T含量的65.3%。大核糖体RNA(lrRNA)和小核糖体RNA(srRNA)的A+T含量分别为72.3%和66.4%。A+T含量最高的是控制区(76.2%)。该线粒体基因组中有15个基因重叠和9个基因间隔区。此外,我们基于襀科11个物种和网襀科2个物种(外类群)的编码蛋白质的基因连接数据集,使用贝叶斯(BI)和最大似然(ML)方法进行了系统发育分析。[物种名称1] + [物种名称2]分支是[物种名称3]的姐妹群,这与传统形态分类一致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dee0/7710318/46a6d273c871/TMDN_A_1674722_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dee0/7710318/46a6d273c871/TMDN_A_1674722_F0001_B.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dee0/7710318/46a6d273c871/TMDN_A_1674722_F0001_B.jpg

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本文引用的文献

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Gene. 2018 Oct 30;675:254-264. doi: 10.1016/j.gene.2018.06.093. Epub 2018 Jun 28.
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Mitochondrial phylogenomics of Hemiptera reveals adaptive innovations driving the diversification of true bugs.半翅目的线粒体系统发育基因组学揭示了推动真蝽多样化的适应性创新。
Proc Biol Sci. 2017 Sep 13;284(1862). doi: 10.1098/rspb.2017.1223.
3
The complete mitochondrial genome of the styloperlid stonefly species Wu (Insecta: Plecoptera) with family-level phylogenetic analyses of the Pteronarcyoidea.
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Capturing the Phylogeny of Holometabola with Mitochondrial Genome Data and Bayesian Site-Heterogeneous Mixture Models.利用线粒体基因组数据和贝叶斯位点异质性混合模型构建全变态昆虫的系统发育树
Genome Biol Evol. 2016 May 22;8(5):1411-26. doi: 10.1093/gbe/evw086.
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