Wu Xiaobo, Zhang Dequan
College of Pharmacy and Chemistry, Dali University, Dali, China.
Institute of Materia Medica, Dali University, Dali, China.
Mitochondrial DNA B Resour. 2019 Oct 11;4(2):3531-3533. doi: 10.1080/23802359.2019.1676678.
Wall., a widely distributed species in southwest China, belongs to a monotypic genus, Wall. in the Scrophulariaceae. To date, there has been no study on its complete chloroplast genome. Hence, we reported the first complete chloroplast genome sequence of . here. The genome sequences of two individuals were extremely similar except slight differences on sequence lengths (152,707 bp and 152,700 bp, respectively). There was a typical circular quadripartite structure. A total of 113 genes were annotated, including 79 protein-coding genes, 29 tRNA genes, four rRNA genes, and one pseudogene. Among the annotated genes, 16 genes contained one intron, whereas another two genes (ycf3 and clpP) possessed two gene introns. Total GC content is 38.1% in the chloroplast genome. Moreover, a total of 63 simple sequence repeats (SSRs) with five types were detected. Phylogenetic analysis revealed that was closely related to L. and Heist. Farbic.
Wall.是中国西南部广泛分布的物种,属于玄参科中一个单型属Wall.。迄今为止,尚未对其完整叶绿体基因组进行研究。因此,我们在此报道了Wall.的首个完整叶绿体基因组序列。两个个体的基因组序列除了在序列长度上有细微差异(分别为152,707 bp和152,700 bp)外极为相似。其具有典型的环状四分体结构。总共注释了113个基因,包括79个蛋白质编码基因、29个tRNA基因、4个rRNA基因和1个假基因。在注释基因中,16个基因含有1个内含子,而另外两个基因(ycf3和clpP)有2个基因内含子。叶绿体基因组的总GC含量为38.1%。此外,共检测到63个具有五种类型的简单序列重复(SSR)。系统发育分析表明,Wall.与L.和Heist. Farbic密切相关。