Ding Chengxiang, Dong Quanmin
Academy of Animal and Veterinary, Qinghai University, Xining, China.
Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau, Xining, Qinghai Province, China.
Mitochondrial DNA B Resour. 2019 Dec 27;5(1):358-359. doi: 10.1080/23802359.2019.1704192.
The complete chloroplast genome from , an important perennial bunchgrass of the Gramineae, is determined in this study. The whole chloroplast genome sequence of has been characterized by Illumina pair-end sequencing. The circular genome is 134,177 bp long, containing a large single-copy region (LSC) of 79,367 bp and a small single-copy region (SSC) of 12,510 bp, which are separated by a pair of 21,150 bp inverted repeat regions (IRs). It encodes a total of 150 genes, including 93 protein-coding genes (75 PCG species), 49 tRNA genes (30 tRNA species) and eight ribosomal RNA genes (4 rRNA species). The most of gene species occur as a single copy, while 30 gene species occur in double copies. The overall A + T content of is 61.6%, while the corresponding values of the LSC, SSC, and IR regions are 63.7, 67.4, and 56.0%, respectively. Phylogenetic analysis suggested that was relatively close to compared with other species of genera in Gramineae among the species analyzed. This complete chloroplast genome will provide valuable insight into conservation and exploitation efforts for this species as well as useful resources for studying the Gramineae phylogeny.
本研究测定了禾本科一种重要的多年生丛生禾草的完整叶绿体基因组。通过Illumina双末端测序对其完整叶绿体基因组序列进行了表征。该环状基因组长度为134,177 bp,包含一个79,367 bp的大单拷贝区域(LSC)和一个12,510 bp的小单拷贝区域(SSC),它们被一对21,150 bp的反向重复区域(IRs)隔开。它总共编码150个基因,包括93个蛋白质编码基因(75种PCG)、49个tRNA基因(30种tRNA)和8个核糖体RNA基因(4种rRNA)。大多数基因种类以单拷贝形式存在,而30种基因种类以双拷贝形式存在。其总体A+T含量为61.6%,而LSC、SSC和IR区域的相应值分别为63.7%、67.4%和56.0%。系统发育分析表明,在所分析的物种中,与禾本科其他属的物种相比,它与 相对较近。这个完整的叶绿体基因组将为该物种的保护和开发工作提供有价值的见解,以及为研究禾本科系统发育提供有用的资源。