1758年林奈所定种尼里-拉菲水牛（偶蹄目：牛科）线粒体全基因组的测定及系统发育分析
Determination and phylogenetic analysis of the complete mitochondrial genome of Linnaeus, 1758 breed Nili-Ravi (Artiodactyla: Bovidae).
作者信息
Lin Qian, Li Meng-Wei, Wang Yan-Zhou, Qiu Hua-Jiao
机构信息
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, PR China.
Buffalo Research Institute, Chinese Academy of Agricultural Sciences and Guangxi Zhuang Nationality Autonomous Region, Nanning, PR China.
出版信息
Mitochondrial DNA B Resour. 2020 Jan 10;5(1):461-462. doi: 10.1080/23802359.2019.1704182.
Nili-Ravi buffalo ( Linnaeus, 1758 breed Nili-Ravi, NRB) is a famous water buffalo breed in the world. It is the first time that the complete mitochondrial genome sequence of the NRB was reported. The total length of the mtDNA is 16,356 bp, It contains the typical structure, including 22 transfer RNA genes, 2 ribosomal RNA genes, 13 protein-coding genes, and 1 non-coding control region (D-loop region). The overall composition of the mtDNA was estimated to be 33.11% for A, 26.45% for T, 26.55% for C, and 13.89% for G. Phylogenetic analyses using neighbor-joining (N-J) computational algorithms showed that the analyzed 18 Ruminantia species are divided into four major clades: Bovidae, Cervidae, Giraffidae, and Atilocapridae. In addition, our work confirmed that NRB has a close genetic relationship with isolate India 4.
尼里-拉菲水牛（林奈，1758年，尼里-拉菲品种，NRB）是世界著名的水牛品种。这是首次报道尼里-拉菲水牛完整的线粒体基因组序列。线粒体DNA（mtDNA）全长16356 bp，具有典型结构，包括22个转运RNA基因、2个核糖体RNA基因、13个蛋白质编码基因和1个非编码控制区（D环区）。mtDNA的总体组成估计为：A占33.11%，T占26.45%，C占26.55%，G占13.89%。使用邻接法（N-J）计算算法进行的系统发育分析表明，所分析的18种反刍动物分为四个主要进化枝：牛科、鹿科、长颈鹿科和叉角羚科。此外，我们的研究证实，尼里-拉菲水牛与印度分离株4具有密切的遗传关系。
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