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复州水牛(偶蹄目:牛科)线粒体全基因组的鉴定与系统发育分析

Identification and phylogenetic analysis of the complete mitochondrial genome of Fuzhong buffalo (Artiodactyla: Bovidae).

作者信息

Li Meng-Wei, Lin Qian, Wang Yan-Zhou, Huang Jia-Xiang, Qiu Hua-Jiao

机构信息

Chinese Academy of Agricultural Sciences and Guangxi Zhuang Nationality Autonomous Region, Buffalo Research Institute, Nanning, China.

Chinese Academy of Agricultural Sciences, Institute of Bast Fiber Crops, Changsha, China.

出版信息

Mitochondrial DNA B Resour. 2020 Jan 20;5(1):713-714. doi: 10.1080/23802359.2020.1714505.

Abstract

Fuzhong buffalo ( Linnaeus, 1758 breed Fuzhong, FB) is one of the famous indigenous breeds of buffalo in China. It is the first time that the complete mitochondrial genome sequence of the FB was reported. The total length of the mtDNA is 16,363 bp, It contains the typical structure, including 22 transfer RNA genes, two ribosomal RNA genes, 13 protein-coding genes and one non-coding control region (D-loop region). The overall composition of the mtDNA was estimated to be 32.98% for A, 26.34% for T, 26.70% for C and 13.98% for G. Phylogenetic analyses using N-J computational algorithms showed that the analyzed 19 ruminantia species are divided into four major clades: Bovidae, Cervidae, Giraffidae and Atilocapridae. In addition, our work confirmed that FB and Murrah buffalo (MB) have a close genetic relationship with fellow tribal members Nili-Ravi buffalo and Mediterranean buffalo. Meanwhile, we also found that FB and MB have a highly similar genetic relationship.

摘要

富钟水牛(林奈,1758年,富钟品种,FB)是中国著名的本土水牛品种之一。这是首次报道富钟水牛的完整线粒体基因组序列。线粒体DNA(mtDNA)全长16363bp,具有典型结构,包括22个转运RNA基因、两个核糖体RNA基因、13个蛋白质编码基因和一个非编码控制区(D环区)。mtDNA的总体组成估计为:A占32.98%,T占26.34%,C占26.70%,G占13.98%。使用N-J计算算法进行的系统发育分析表明,所分析的19种反刍动物分为四个主要进化枝:牛科、鹿科、长颈鹿科和叉角羚科。此外,我们的研究证实,富钟水牛和摩拉水牛(MB)与同属的尼里-拉菲水牛和地中海水牛有密切的遗传关系。同时,我们还发现富钟水牛和摩拉水牛有高度相似的遗传关系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e551/7748632/84e8c529d9f1/TMDN_A_1714505_F0001_B.jpg

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