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(某物种)的完整叶绿体基因组及其系统发育意义。 (括号内内容缺失,需补充完整物种名)

The complete chloroplast genome of () and its phylogenetic implication.

作者信息

Cheng Hong, Kong Wei-Ping, Zhang Min-Min, Hou Dong

机构信息

Gansu Academy of Agricultural Sciences, Vegetable Research Institute, Lanzhou, China.

Key Laboratory of Fruit Breeding Technology, Ministry of Agriculture and Rural Affairs, Zhengzhou, China.

出版信息

Mitochondrial DNA B Resour. 2020 Jan 16;5(1):654-655. doi: 10.1080/23802359.2019.1711231.

Abstract

The genus contains 52 species, including two economically significant crops, cucumber and melon, as well as other important species. is a wild relative of , native to Africa. is rich in vitamins and minerals in gherkin fruits and carries broad-spectrum resistance to multiplex biotic and abiotic stress, such as powdery mildew, fusarium wilt, and meloidogyn incognita. provides a valuable gene pool for crop improvement of crops. In this study, the complete chloroplast (cp) genome sequence of was determined using next-generation sequencing. The entire cp genome was determined to be 156,577 bp in length. It contained large single-copy (LSC) and small single-copy (SSC) regions of 85,971 and 18,100 bp, respectively, which were separated by a pair of 26,253 bp inverted repeat (IR) regions. The genome contained 134 genes, including 88 protein-coding genes, 37 tRNA genes, 8 rRNA genes and 1 pseudogene infA. The overall GC content of the genome is 37.0%. A phylogenetic tree reconstructed by 48 chloroplast genomes reveals that is a separate branch in .

摘要

该属包含52个物种,其中包括两种具有重要经济价值的作物——黄瓜和甜瓜,以及其他重要物种。[物种名称1]是[物种名称2]的野生近缘种,原产于非洲。[物种名称1]的小黄瓜果实富含维生素和矿物质,并对多种生物和非生物胁迫具有广谱抗性,如白粉病、枯萎病和南方根结线虫。[物种名称1]为[物种名称2]作物的改良提供了宝贵的基因库。在本研究中,利用二代测序技术测定了[物种名称1]的完整叶绿体(cp)基因组序列。整个cp基因组长度为156,577 bp。它包含长度分别为85,971和18,100 bp的大单拷贝(LSC)和小单拷贝(SSC)区域,这两个区域被一对26,253 bp的反向重复(IR)区域隔开。该基因组包含134个基因,其中包括88个蛋白质编码基因、37个tRNA基因、8个rRNA基因和1个假基因infA。基因组的总体GC含量为37.0%。由48个叶绿体基因组重建的系统发育树表明,[物种名称1]在[某分类层级]中是一个独立的分支。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9b9a/7748529/81247c83c0a5/TMDN_A_1711231_F0001_B.jpg

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