Kumar Pankaj, Aeron Abhinav, Shaw Niru, Singh Ajay, Bajpai V K, Pant Shailja, Dubey Ramesh Chandra
Department of Microbiology, Dolphin (PG) College of Science and Agriculture, Chunni Kalan, Fatehgarh Sahib, Chandigarh, 140307, Punjab, India.
Department of Microbiology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Dehradun, 248007, Uttarakhand, India.
Heliyon. 2020 Dec 15;6(12):e05701. doi: 10.1016/j.heliyon.2020.e05701. eCollection 2020 Dec.
Three potential rhizobacteria namely (MTCC 10216), sp. (MTCC 9002) and (MTCC 8528) procured from IMTECH, Chandigarh (India) were evaluated individually and as consortia for its phosphate (P) solubilizing ability and effect of growth of fenugreek ( L.) and tomato ( L.). Phosphate solubilizing ability of these strains individually and as consortia was tested on Pikovskayas agar medium, Phosphate solubilizing agar medium and National Botanical Research Institute phosphate agar medium containing six different sources of insoluble inorganic phosphate such as tri-calcium phosphate (TCP), di-calcium phosphate (DCP), zinc phosphate (ZP), ferric phosphate (FP), sodium di-hydrogen phosphate (SP), and aluminum phosphate (AP), and two organic P such as calcium and sodium phytate. The maximum P solubilizing ability was recorded in consortium-4 having all three potential bacterial strains. Phosphate solubilization after 7 day of incubation was 37.9 mg/100 ml of TCP, 40.01 mg/100 ml of DCP, 15.79 mg/100 ml of FP, 43.02 mg/100 ml of SP, no solubilization of ZP and AP, 39.75 mg/100 ml of calcium phytate and 24.01mg/100 ml of sodium phytate. Seed germination and the other plant parameters such as plant height and weight significantly increased in fenugreek and tomato seeds, bio-primed with consortium-4 followed by consortium-3. After bio-priming of seeds in pot assay, the level of phosphorus in soil got increased by 54% in consortium-4 treated soil followed by consortium-3 (47%) over untreated control soil. Based on these findings, consoritium-4 could be recommended as a good bio-inoculant for fenugreek, tomato and other crops in comparison to individual strains and other consortia.
从印度昌迪加尔的微生物技术研究所（IMTECH）获得的三种潜在根际细菌，即（MTCC 10216）、 属（MTCC 9002）和 （MTCC 8528），分别以及作为混合菌群对其解磷能力和对胡芦巴（ L.）和番茄（ L.）生长的影响进行了评估。这些菌株分别以及作为混合菌群的解磷能力在含有六种不同来源不溶性无机磷（如磷酸三钙（TCP）、磷酸二钙（DCP）、磷酸锌（ZP）、磷酸铁（FP）、磷酸二氢钠（SP）和磷酸铝（AP））以及两种有机磷（如植酸钙和植酸钠）的皮科夫斯卡娅琼脂培养基、解磷琼脂培养基和国家植物研究所磷酸盐琼脂培养基上进行了测试。在含有所有三种潜在细菌菌株的混合菌群4中记录到最大解磷能力。培养7天后，TCP的解磷量为37.9毫克/100毫升，DCP为40.01毫克/100毫升，FP为15.79毫克/100毫升，SP为43.02毫克/100毫升，ZP和AP未溶解，植酸钙为39.75毫克/100毫升，植酸钠为24.01毫克/100毫升。用混合菌群4然后是混合菌群3进行生物引发处理后，胡芦巴和番茄种子的发芽率以及其他植物参数（如株高和重量）显著增加。在盆栽试验中对种子进行生物引发处理后，混合菌群4处理的土壤中磷含量比未处理的对照土壤增加了54%，其次是混合菌群3（47%）。基于这些发现，与单个菌株和其他混合菌群相比，混合菌群4可被推荐为胡芦巴、番茄和其他作物的良好生物接种剂。
