西红花提取物对链脲佐菌素诱导的糖尿病大鼠视网膜的保护抗氧化作用。

Protective antioxidant effects of saffron extract on retinas of streptozotocin-induced diabetic rats.

机构信息

Ophthalmology Clinic, Department of Medicine, University of Patras, Rio, Greece.

Laboratory of Human and Animal Physiology, Department of Biology, University of Patras, Patras, Greece.

出版信息

Rom J Ophthalmol. 2020 Oct-Dec;64(4):394-403. doi: 10.22336/rjo.2020.61.

DOI:10.22336/rjo.2020.61

PMID:33367177

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7739020/

Abstract

Oxidative stress plays an important role in the pathogenesis of diabetic retinopathy. The aim of the present study was to investigate the effect of Crocus sativus L. styles (saffron) extract on oxidative stress indices of retina in streptozotocin (STZ)-induced diabetic rats. Adult male Wistar rats (n=20) were randomized into the following 4 groups (n=6-7/ group): Control group (C): normal, Control + Saffron group (CS): non-diabetic rats treated with 60 mg/ kg of saffron extract, Diabetic group (D) and Diabetic + Saffron group (DS): diabetic rats treated with 60 mg/ kg saffron extract. We determined the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) as markers of antioxidant response, as well as malondialdehyde (MDA) as a marker of lipid peroxidation. Induction of diabetes caused a significant decline in the activities of CAT (76.43%), SOD (53.43%) and GPx (77.58%). MDA levels were significantly lower in the DS group (0.878 ± 0.375 nmol MDA/ mg protein) as compared to D group (1.950 ± 0.299 nmol MDA/ mg protein, p<0.01) and in the CS group (0.503 ± 0.221) in comparison to C group (1.699 ± 0.454, p<0.01). Moreover, SOD and GPx activities were significantly higher (more than 1.5 and 3.5-fold respectively) after treatment with saffron (p<0.01). Regarding the retinas of non-diabetic animals, the administration of the extract caused an > 1.8-fold increase in the activity of CAT (p<0.05) and a 3-fold decrease in MDA levels (p<0.01). This study showed that saffron extract has a protective antioxidant action in retinas of diabetic rats. C = Control group, CS = non-diabetic rats diabetic rats treated with 60 mg/ kg saffron extract, D = diabetic group, DS = diabetic rats treated with 60 mg/ kg saffron extract, SOD = superoxide dismutase, GPx = glutathione peroxidase, CAT = catalase, MDA = malondialdehyde, DM = diabetes mellitus, DR = diabetic retinopathy, ROS = reactive oxygen species, STZ = streptozotocin, GSH = reduced glutathione.

摘要

氧化应激在糖尿病性视网膜病变的发病机制中起着重要作用。本研究旨在探讨藏红花（藏红花）提取物对链脲佐菌素（STZ）诱导的糖尿病大鼠视网膜氧化应激指标的影响。

成年雄性 Wistar 大鼠（n=20）随机分为以下 4 组（n=6-7/组）：对照组（C）：正常，对照组+藏红花组（CS）：非糖尿病大鼠用 60mg/kg 藏红花提取物治疗，糖尿病组（D）和糖尿病+藏红花组（DS）：糖尿病大鼠用 60mg/kg 藏红花提取物治疗。我们测定了超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）的活性作为抗氧化反应的标志物，以及丙二醛（MDA）作为脂质过氧化的标志物。糖尿病的诱导导致 CAT（76.43%）、SOD（53.43%）和 GPx（77.58%）的活性显著下降。与 D 组（1.950±0.299 nmol MDA/mg 蛋白）和 CS 组（0.503±0.221）相比，DS 组的 MDA 水平显著降低（0.878±0.375 nmol MDA/mg 蛋白，p<0.01）。此外，藏红花治疗后 SOD 和 GPx 活性显著升高（分别升高 1.5 倍和 3.5 倍以上，p<0.01）。关于非糖尿病动物的视网膜，提取物的给药导致 CAT 活性增加了 1.8 倍以上（p<0.05），MDA 水平降低了 3 倍（p<0.01）。

这项研究表明，藏红花提取物对糖尿病大鼠的视网膜具有保护抗氧化作用。C=对照组，CS=非糖尿病大鼠用 60mg/kg 藏红花提取物治疗，D=糖尿病组，DS=糖尿病大鼠用 60mg/kg 藏红花提取物治疗，SOD=超氧化物歧化酶，GPx=谷胱甘肽过氧化物酶，CAT=过氧化氢酶，MDA=丙二醛，DM=糖尿病，DR=糖尿病性视网膜病变，ROS=活性氧，STZ=链脲佐菌素，GSH=还原型谷胱甘肽。