Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Turkey.
Department of Biochemistry, Faculty of Veterinary Medicine, Atatürk University, Erzurum, Turkey.
J Trace Elem Med Biol. 2023 Dec;80:127315. doi: 10.1016/j.jtemb.2023.127315. Epub 2023 Sep 29.
Heavy metals are one of the environmental pollutants. Lead (Pb) is one of the most common of these heavy metals. In this study, it was aimed at investigating the effects of syringic acid (SA) against testicular toxicity in rats administered lead acetate (PbAc).
In the present study, a total of 35 Sprague-Dawley rats, 7 in each group, were used. The rats were divided into 5 groups, with 7 male rats in each group. Rats were given PbAc and SA orally for 7 days. The effects of PbAc and SA on epididymal sperm quality and apoptosis, inflammation, oxidative stress and histopathological changes in testicular tissue were determined.
While PbAc disrupted the seminiferous tubules and produced atrophic images, SA corrected these histological abnormalities. PbAc adminisration significantly reduced the levels of SOD, GSH, GPx, CAT, NRF-2 and NQO1 and significantly increased the levels of MDA and 8-OHdG in the testicular tissue of rats, while SA improved this situation. NF-κB, TNF-α, IL-1β, NLRP3, RAGE, ATF6, PERK, IRE1, CHOP, and GRP78 genes expression levels increased with PbAc administration, however these levels decreased with SA administration. In addition, PbAc increased the levels of apoptotic markers Bax, Caspase-3 and APAF-1 and decreased the level of Bcl-2, while SA improved this situation. It was observed that PbAc significantly reduced sperm quality in rats, while SA positively affected sperm quality.
As a result, SA administered against PbAc-induced testicular dysfunction in rats can provide effective protection at doses of 25 mg/kg/bw and 50 mg/kg/bw.
重金属是环境污染物之一。铅（Pb）是这些重金属中最常见的一种。在这项研究中，旨在研究丁香酸（SA）对醋酸铅（PbAc）给药大鼠睾丸毒性的影响。
本研究共使用 35 只 Sprague-Dawley 大鼠，每组 7 只。将大鼠分为 5 组，每组 7 只雄性大鼠。大鼠口服给予 PbAc 和 SA 共 7 天。确定 PbAc 和 SA 对附睾精子质量和凋亡、炎症、氧化应激以及睾丸组织的组织病理学变化的影响。
虽然 PbAc 破坏了曲细精管并产生了萎缩图像，但 SA 纠正了这些组织学异常。PbAc 给药显著降低了大鼠睾丸组织中超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GPx）、过氧化氢酶（CAT）、核因子红细胞 2 相关因子 2（NRF-2）和醌氧化还原酶 1（NQO1）的水平，并显著增加了丙二醛（MDA）和 8-羟基脱氧鸟苷（8-OHdG）的水平，而 SA 改善了这种情况。NF-κB、肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、NLRP3、RELA、激活转录因子 6（ATF6）、蛋白激酶 RNA 样内质网激酶（PERK）、肌醇需求酶 1（IRE1）、C/EBP 同源蛋白（CHOP）和葡萄糖调节蛋白 78（GRP78）基因表达水平随着 PbAc 给药而增加，而随着 SA 给药而降低。此外，PbAc 增加了凋亡标志物 Bax、Caspase-3 和 APAF-1 的水平，降低了 Bcl-2 的水平，而 SA 改善了这种情况。观察到 PbAc 显著降低了大鼠的精子质量，而 SA 则对精子质量产生了积极影响。
因此，在 25mg/kg/bw 和 50mg/kg/bw 剂量下，SA 对醋酸铅（PbAc）诱导的大鼠睾丸功能障碍具有有效保护作用。
