Qurollo Barbara A, Stillman Brett A, Beall Melissa J, Foster Paulette, Hegarty Barbara C, Breitschwerdt Edward B, Chandrashekar Ramaswamy
Am J Vet Res. 2021 Jan;82(1):71-80. doi: 10.2460/ajvr.82.1.71.
To compare the performance of 5 synthetic peptide-based ELISAs with that of 3 commercially available immunofluorescent assays (IFAs) for serologic diagnosis of anaplasmosis and ehrlichiosis in dogs.
A convenience set of 109 serum samples obtained before and at various times after inoculation for 23 dogs that were experimentally infected with , or and 1 uninfected control dog in previous studies.
All serum samples were assessed with 5 synthetic peptide-based ELISAs designed to detect antibodies against , and and 3 whole organism-based IFAs designed to detect antibodies against , and The species-specific seroreactivity, cross-reactivity with the other tick-borne pathogens (TBPs), and diagnostic sensitivity and specificity were calculated for each assay and compared among assays.
All serum samples obtained from dogs experimentally infected with a TBP yielded positive results on a serologic assay specific for that pathogen. In general, sensitivity was comparable between ELISAs and IFAs and tended to increase with duration after inoculation. Compared with the IFAs, the corresponding ELISAs were highly specific and rarely cross-reacted with antibodies against other TBPs.
Results suggested that peptide-based ELISAs had enhanced specificity relative to whole organism-based IFAs for detection of antibodies against and spp, which should facilitate accurate diagnosis and may help detect dogs coinfected with multiple TBPs.
比较5种基于合成肽的酶联免疫吸附测定（ELISA）与3种市售免疫荧光测定（IFA）对犬无形体病和埃立克体病进行血清学诊断的性能。
从23只在先前研究中经实验感染了[病原体名称]、[病原体名称]或[病原体名称]的犬以及1只未感染的对照犬接种前后不同时间获得的109份血清样本组成的便利样本集。
所有血清样本均采用5种旨在检测抗[病原体名称]、[病原体名称]和[病原体名称]抗体的基于合成肽的ELISA以及3种旨在检测抗[病原体名称]、[病原体名称]和[病原体名称]抗体的基于全生物体的IFA进行评估。计算每种测定的种属特异性血清反应性、与其他蜱传病原体（TBP）的交叉反应性以及诊断敏感性和特异性，并在各测定之间进行比较。
从经实验感染TBP的犬获得的所有血清样本在针对该病原体的血清学测定中均产生阳性结果。一般来说，ELISA和IFA之间的敏感性相当，并且倾向于随着接种后的时间延长而增加。与IFA相比，相应的ELISA具有高度特异性，很少与针对其他TBP的抗体发生交叉反应。
结果表明，基于肽的ELISA相对于基于全生物体的IFA在检测抗[病原体名称]和[病原体名称] spp抗体方面具有更高的特异性，这应有助于准确诊断，并可能有助于检测同时感染多种TBP的犬。
