Patel Jignesh G, Luo Tian, Zhang Xiaofeng, McBride Jere W
Department of Pathology, University of Texas Medical Branch, Galveston, TX, United States.
Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, United States.
Front Vet Sci. 2024 Oct 21;11:1481934. doi: 10.3389/fvets.2024.1481934. eCollection 2024.
is the primary etiologic agent of canine monocytic ehrlichiosis, a serious and sometimes fatal hemorrhagic disease of dogs. Diagnosis of infection is often retrospectively confirmed by serologic detection of antibodies by immunofluorescent microscopy. Our laboratory previously identified numerous major immunoreactive proteins with species-specific linear antibody epitopes that are useful for immunodiagnosis of CME. More recently, we have defined the entire antibody-reactive immunome of , substantially increasing the number of major immunoreactive proteins known to exist. In this study, we analyzed and compared seven recently identified antibody reactive proteins with established diagnostic antigens including tandem repeat proteins TRP19, TRP36 and TRP140 and observed comparable immunoreactivity. Many of these proteins were conserved in different strains. Multiple linear antibody epitopes were mapped in a highly conserved TRP (Ecaj_0126), including within the tandem repeat domain. Temporal antibody responses were examined, and multiple proteins reacted with antibodies in sera as early as 21 days post experimental infection. Host-specific expression of the proteins was examined which revealed that some proteins exhibited higher expression in mammalian cells, while others in tick cells. This study has identified new immunodiagnostic candidates that exhibit different host expression patterns, information which may be useful for developing ultrasensitive immunodiagnostics and effective vaccines for CME.
是犬单核细胞埃立希体病的主要病原体，犬单核细胞埃立希体病是一种严重的、有时会致命的犬类出血性疾病。感染的诊断通常通过免疫荧光显微镜血清学检测抗体来进行回顾性确认。我们实验室之前鉴定出了许多具有物种特异性线性抗体表位的主要免疫反应蛋白，这些蛋白可用于犬单核细胞埃立希体病的免疫诊断。最近，我们确定了的整个抗体反应免疫组，大大增加了已知存在的主要免疫反应蛋白的数量。在本研究中，我们分析并比较了最近鉴定出的七种抗体反应蛋白与已确立的诊断抗原，包括串联重复蛋白TRP19、TRP36和TRP140，并观察到了相当的免疫反应性。这些蛋白中的许多在不同菌株中是保守的。在一个高度保守的TRP（Ecaj_0126）中绘制了多个线性抗体表位，包括在串联重复结构域内。检测了时间性抗体反应，早在实验感染后21天，多种蛋白就与血清中的抗体发生反应。检测了这些蛋白的宿主特异性表达，结果显示一些蛋白在哺乳动物细胞中表达较高，而另一些在蜱细胞中表达较高。本研究鉴定出了表现出不同宿主表达模式的新的免疫诊断候选物，这些信息可能有助于开发用于犬单核细胞埃立希体病的超灵敏免疫诊断方法和有效的疫苗。
