Department of Biology, Kunsan National University , Gunsan, Republic of Korea.
Plant Signal Behav. 2021 Mar 4;16(3):1868131. doi: 10.1080/15592324.2020.1868131. Epub 2020 Dec 28.
Submergence and waterlogging lead to significant reductions in crop productivity and trigger dramatic changes in gene expression of plant biotic/abiotic stress response. Several of the host factors are involved in low-oxygen stress that is induced by endogenous reactive oxygen species (ROS) accumulation. ypoxia-response nknown rotein (HUP) has been found as a host factor of hypoxia screening but HUPs function largely is unknown. In this study, we found the Arabidopsis gene which was conserved in different plant species and responded to various oxidative stress. promoter analysis showed GUS activity in root and leaf tissues was significantly responsive to oxidative stress. HUP26-GFP is predominantly located in the cytoplasmic region. overexpression results in altered enhanced gene expression and reduced ion leakage levels compared with knockout and WT plants after inoculation with DC3000. overexpression transgenic plants showed improved resistance to DC3000, but knockout plants exhibited increased susceptibility. Collectively, these results indicate that HUP26 plays important role in responses to various oxidative stress and confers biotic stress resistance. Engineering of gene expression may represent a strategy to enhance biotic stress resistance of crops.
淹没和积水会导致作物生产力显著下降，并引发植物生物/非生物胁迫反应的基因表达的剧烈变化。一些宿主因子参与由内源性活性氧（ROS）积累引起的低氧胁迫。缺氧反应未知蛋白（HUP）已被发现作为缺氧筛选的宿主因子，但 HUP 的功能在很大程度上是未知的。在这项研究中，我们发现了拟南芥基因，该基因在不同的植物物种中保守，对各种氧化应激有反应。启动子分析表明，GUS 活性在根和叶组织中对氧化应激有明显的响应。HUP26-GFP 主要位于细胞质区域。与 敲除和 WT 植物相比，过表达后，基因的表达增强，离子渗漏水平降低，接种 DC3000 后。过表达转基因植物对 DC3000 的抗性提高，但 敲除植物的敏感性增加。总的来说，这些结果表明 HUP26 在应对各种氧化应激中发挥重要作用，并赋予生物胁迫抗性。该基因表达的工程化可能代表一种提高作物生物胁迫抗性的策略。
