New function of Hypoxia-responsive unknown protein in enhanced resistance to biotic stress.

Submergence and waterlogging lead to significant reductions in crop productivity and trigger dramatic changes in gene expression of plant biotic/abiotic stress response. Several of the host factors are involved in low-oxygen stress that is induced by endogenous reactive oxygen species (ROS) accumulation. ypoxia-response nknown rotein (HUP) has been found as a host factor of hypoxia screening but HUPs function largely is unknown. In this study, we found the Arabidopsis gene which was conserved in different plant species and responded to various oxidative stress. promoter analysis showed GUS activity in root and leaf tissues was significantly responsive to oxidative stress. HUP26-GFP is predominantly located in the cytoplasmic region. overexpression results in altered enhanced gene expression and reduced ion leakage levels compared with knockout and WT plants after inoculation with DC3000. overexpression transgenic plants showed improved resistance to DC3000, but knockout plants exhibited increased susceptibility. Collectively, these results indicate that HUP26 plays important role in responses to various oxidative stress and confers biotic stress resistance. Engineering of gene expression may represent a strategy to enhance biotic stress resistance of crops.