Hadish John A, Hargarten Heidi L, Zhang Huiting, Mattheis James P, Ficklin Stephen P, Honaas Loren A
Molecular Plant Science Program Washington State University Pullman WA USA.
Department of Horticulture Washington State University Pullman WA USA.
Plant Direct. 2024 Dec 20;8(12):e70025. doi: 10.1002/pld3.70025. eCollection 2024 Dec.
Research on how plants respond to hypoxia has concentrated on model organisms where tissues can only survive hypoxic conditions for a few hours to a few days. In contrast, hypoxic conditions are used commercially as a method to prolong the shelf life of (apple) fruit for up to a year of storage without substantial changes in fruit quality, not to mention a lack of tissue death. This ability of apples to withstand protracted hypoxic conditions is an interesting adaptation that has had limited molecular investigation despite its economic importance. Here, we investigate the long-term apple hypoxia response using a time-course RNA-seq analysis of several postharvest storage conditions. We use phylogenetics, differential expression, and regulatory networks to identify genes that regulate and are regulated by the hypoxia response. We identify potential neofunctionalization of core-hypoxia response genes in apples, including novel regulation of group VII ethylene response factor (ERF VII) and plant cysteine oxidase (PCO) family members.
关于植物如何应对缺氧的研究主要集中在模式生物上，这些生物的组织在缺氧条件下只能存活数小时至数天。相比之下，缺氧条件在商业上被用作一种延长（苹果）果实货架期的方法，可储存长达一年，果实品质无显著变化，更不用说组织死亡了。苹果这种耐受长期缺氧条件的能力是一种有趣的适应性变化，尽管其具有经济重要性，但在分子层面的研究却很有限。在这里，我们通过对几种采后储存条件进行时间进程RNA测序分析，来研究苹果对缺氧的长期反应。我们利用系统发育学、差异表达和调控网络来鉴定调控缺氧反应以及受缺氧反应调控的基因。我们确定了苹果中核心缺氧反应基因的潜在新功能化，包括对VII组乙烯反应因子（ERF VII）和植物半胱氨酸氧化酶（PCO）家族成员的新调控。
