Centre for Cell Biology and Cutaneous Research, The Blizard Institute, Queen Mary University of London, London, UK.
Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
Br J Dermatol. 2021 Jul;185(1):26-35. doi: 10.1111/bjd.19760. Epub 2021 Mar 1.
Tape strips have been used widely in dermatology research as a minimally invasive method to sample the epidermis, avoiding the need for skin biopsies. Most research has focused on epidermal pathology, such as atopic eczema, but there is increasing research into the use of tape strips in other dermatoses, such as skin cancer, and the microbiome. This review summarizes the technique of tape stripping, and discusses which dermatoses have been studied by tape stripping and alternative minimally invasive sampling methods. We review the number of tape strips needed from each patient and the components of the epidermis that can be obtained by tape stripping. With a focus on protein and RNA extraction, we address the techniques used to process tape strips. There is no optimal protocol to extract protein, as this depends on the abundance of the protein studied, its level of expression in the epidermis and its solubility. Many variables can alter the amount of protein obtained from tape strips, which must be standardized to ensure consistency between samples. No study has compared different RNA extraction techniques, but our own experience is that RNA yield is optimized by using 20 tape strips and the use of a cell scraper.
胶带条已广泛应用于皮肤科研究，作为一种微创方法来取样表皮，避免了皮肤活检的需要。大多数研究都集中在表皮病理学上，如特应性皮炎，但越来越多的研究关注胶带条在其他皮肤病中的应用，如皮肤癌和微生物组。这篇综述总结了胶带条的技术，讨论了哪些皮肤病已经通过胶带条和其他微创取样方法进行了研究。我们回顾了每位患者需要的胶带条数量以及胶带条可以获得的表皮成分。重点介绍蛋白质和 RNA 提取，我们介绍了用于处理胶带条的技术。没有一种提取蛋白质的最佳方案，因为这取决于研究的蛋白质的丰度、其在表皮中的表达水平及其可溶性。许多变量会改变从胶带条中获得的蛋白质的数量，这必须标准化，以确保样品之间的一致性。没有研究比较过不同的 RNA 提取技术，但我们自己的经验是，使用 20 个胶带条和细胞刮刀可以优化 RNA 的产量。
