血浆 miR-1247-5p、miR-301b-3p 和 miR-105-5p 作为非小细胞肺癌早期诊断的潜在生物标志物。
Plasma miR-1247-5p, miR-301b-3p and miR-105-5p as potential biomarkers for early diagnosis of non-small cell lung cancer.
机构信息
Department of Clinical Laboratory, Shandong Cancer Hospital and Institute, Shandong First Medical University and Shandong Academy of Medical Sciences, Shandong, China.
Shandong First Medical University and Shandong Academy of Medical Sciences, Shandong, China.
出版信息
Thorac Cancer. 2021 Feb;12(4):539-548. doi: 10.1111/1759-7714.13800. Epub 2020 Dec 28.
BACKGROUND
Accumulating evidence shows that microRNAs are aberrantly expressed and exert essential roles in the tumorigenesis and tumor progression of non-small cell lung cancer (NSCLC).
METHODS
The plasma miRNAs from five healthy donors and four NSCLC patients were profiled by miRNA microarray. The differentially expressed miRNAs from 154 primary NSCLC patients and 146 healthy donors were subjected to RNA isolation and verified by quantitative PCR (qPCR).
RESULTS
The miRNA microarray analysis revealed that 40 differential miRNAs between NSCLC patients and healthy donors were selected. We found that the plasma miR-1247-5p, miR-301b-3p and miR-105-5p levels of patients were significantly higher than those of healthy controls. The receiver operating characteristic curve (ROC) analyses revealed higher area under the ROC curve (AUC) values and higher sensitivity/specificity of carcinoembryonic antigen (CEA) in combination with miR-1247-5p, miR-301b-3p, or miR-105-5p were superior to that of CEA alone.
CONCLUSIONS
High miR-1247-5p, miR-301b-3p and miR-105-5p expression have been demonstrated to accelerate tumorigenesis, and these three miRNAs in plasma act as novel biomarkers for the early diagnosis of NSCLC patients.
KEY POINTS
Plasma miR-1247-5p, miR-301b-3p and miR-105-5p act as novel biomarkers for early NSCLC and NSCLC.
背景
越来越多的证据表明，miRNAs 在非小细胞肺癌（NSCLC）的发生和发展中表达异常，并发挥着重要作用。
方法
采用 miRNA 微阵列分析了 5 名健康供体和 4 名 NSCLC 患者的血浆 miRNAs。对 154 例原发性 NSCLC 患者和 146 例健康供体的差异表达 miRNAs 进行 RNA 分离，并通过定量 PCR（qPCR）进行验证。
结果
miRNA 微阵列分析显示，NSCLC 患者和健康供体之间有 40 个差异 miRNA。我们发现患者的血浆 miR-1247-5p、miR-301b-3p 和 miR-105-5p 水平明显高于健康对照组。受试者工作特征曲线（ROC）分析显示，癌胚抗原（CEA）联合 miR-1247-5p、miR-301b-3p 或 miR-105-5p 的 ROC 曲线下面积（AUC）值较高，敏感性/特异性较高，优于单独 CEA。
结论
高表达 miR-1247-5p、miR-301b-3p 和 miR-105-5p 已被证明可加速肿瘤发生，这三种血浆 miRNA 可作为 NSCLC 患者早期诊断的新型生物标志物。
关键点
血浆 miR-1247-5p、miR-301b-3p 和 miR-105-5p 可作为早期 NSCLC 和 NSCLC 的新型生物标志物。
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