Dermatology Department, Hospital Central "Dr. Ignacio Morones Prieto", Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico.
Gac Med Mex. 2020;156(5):418-423. doi: 10.24875/GMM.M20000430.
The use of soap for skin cleansing is common among the population. However, it is possible that it causes damage to skin cells and disrupts the skin barrier.
To determine the cytotoxic effect of soaps on in vitro-cultured keratinocytes and to correlate it with clinical irritation.
A survey was conducted to find out the most widely used commercial soaps and their number. Subsequently, their cytotoxicity was evaluated in human keratinocyte cultures using the resazurin assay. The soaps with the highest and lowest cytotoxicity were applied to the skin of healthy volunteers to assess their effect on the skin barrier using colorimetry and transepidermal water loss (TEWL) assays.
Of the analyzed soaps, 37 % were shown to be toxic to keratinocytes in vitro. The soap with the highest toxicity induced the highest rate of erythema and TEWL, in comparison with the least toxic soap and the vehicle used as the control solution.
Soaps marketed for skin cleansing can contain chemical ingredients that damage human keratinocytes and cause skin barrier subclinical irritation. Their use can worsen preexisting dermatoses, generate xerotic or irritant contact dermatitis, and cause atrophy and dermatoporosis.
皮肤清洁用肥皂在人群中很常见。然而，它有可能对皮肤细胞造成损害并破坏皮肤屏障。
确定肥皂对体外培养的角质细胞的细胞毒性作用，并将其与临床刺激相关联。
进行了一项调查，以找出最广泛使用的商业肥皂及其数量。随后，使用 Resazurin 测定法在人角质细胞培养物中评估其细胞毒性。将具有最高和最低细胞毒性的肥皂应用于健康志愿者的皮肤，使用色度计和经皮水分流失（TEWL）测定法评估其对皮肤屏障的影响。
在所分析的肥皂中，有 37%显示出对体外角质细胞具有毒性。与毒性最低的肥皂和用作对照溶液的载体相比，毒性最高的肥皂诱导出最高的红斑和 TEWL 率。
用于皮肤清洁的市售肥皂可能含有损害人类角质细胞并引起皮肤屏障亚临床刺激的化学物质。它们的使用会使已存在的皮肤病恶化，产生干燥或刺激性接触性皮炎，并导致萎缩和皮肤骨质疏松症。
