Peanut Research Institute, College of Agronomy, Shenyang Agricultural University, Shenyang, China.
DNA Cell Biol. 2021 Feb;40(2):373-383. doi: 10.1089/dna.2020.6245. Epub 2020 Dec 29.
Peanut is an important crash crop worldwide, and it is often threatened by drought stress due to unexpected extreme weather events. In this work, NH5 and FH18 were selected as drought-tolerant and drought-sensitive varieties, respectively. Comparison of their physiological responses revealed that NH5 showed less wilting, higher relative water content and lower water loss rate of detached leaves, lower electrolyte leakage, and stronger antioxidant ability under drought stress than did FH18. Based on comparative transcriptomic analysis, 5376 differentially expressed mRNAs were commonly identified in the two varieties, and 2993 genes specifically changed in the drought-tolerant variety and were mainly enriched in photosynthesis-antenna proteins and photosynthetic pathways. Furthermore, 73 microRNAs (miRNAs) were differentially expressed in the drought tolerance variety specifically under drought stress; of these, two key candidate miRNAs, novel miR_416 and novel miR_73, were identified, and the majority of their target genes were enriched in phenylpropanoid biosynthesis, linoleic acid metabolism, and cutin, suberine, and wax biosynthesis. This study lays the foundation for the analysis of the molecular mechanism of drought tolerance and promotes the genetic improvement of peanut drought tolerance.
花生是世界范围内的一种重要作物，由于意外的极端天气事件，常受到干旱胁迫的威胁。在这项工作中，NH5 和 FH18 分别被选为耐旱和耐旱敏感品种。对它们生理响应的比较表明，与 FH18 相比，NH5 在干旱胁迫下表现出较少的萎蔫、更高的相对水含量和更低的离体叶片失水率、更低的电解质泄漏率和更强的抗氧化能力。基于比较转录组分析，在两个品种中共同鉴定出 5376 个差异表达的 mRNAs，2993 个基因在耐旱品种中特异性变化，主要富集在光合作用天线蛋白和光合作用途径中。此外，在耐旱品种中，73 个 microRNAs（miRNAs）在干旱胁迫下特异性表达；其中，两个关键候选 miRNA，novel miR_416 和 novel miR_73，被鉴定出来，它们的大多数靶基因富集在苯丙烷生物合成、亚油酸代谢、角质、栓质和蜡生物合成中。本研究为耐旱性的分子机制分析奠定了基础，并促进了花生耐旱性的遗传改良。
