Zhao Xiaobo, Li Chunjuan, Wan Shubo, Zhang Tingting, Yan Caixia, Shan Shihua
Laboratory of Genetics and Breeding, Shandong Peanut Research Institute, Qingdao, 266100, Shandong Province, People's Republic of China.
Shandong Academy of Agricultural Sciences, Jinan, 250100, Shandong Province, People's Republic of China.
Mol Biol Rep. 2018 Apr;45(2):119-131. doi: 10.1007/s11033-018-4145-4. Epub 2018 Jan 12.
The peanut (Arachis hypogaea) is an important crop species that is threatened by drought stress. The genome sequences of peanut, which was officially released in 2016, may help explain the molecular mechanisms that underlie drought tolerance in this species. We report here a gene expression profiling of A. hypogaea to gain a global view of its drought resistance. Using whole-transcriptome sequencing, we analysed differential gene expression in response to drought stress in the drought-resistant peanut cultivar J11. Pooled samples obtained at 6, 12, 18, 24, and 48 h were compared with control samples at 0 h. In total, 51,554 genes were found, including 49,289 known genes and 2265 unknown genes. We identified 224 differentially expressed transcription factors, 296,335 SNPs and 28,391 InDELs. In addition, we detected significant differences in the gene expression profiles of the treatment and control groups. After comparing the two groups, 4648 genes were identified. An in-depth analysis of the data revealed that a large number of genes were associated with drought stress, including transcription factors and genes involved in photosynthesis-antenna proteins, carbon metabolism and the citrate cycle. The results of this study provide insights into the diverse mechanisms that underlie the successful establishment of drought resistance in the peanut, thereby facilitating the identification of important genes in the peanut related to drought management. Transcriptome analysis based on RNA-Seq is a powerful approach for gene discovery and molecular marker development for this species.
花生（Arachis hypogaea）是一种重要的作物品种，受到干旱胁迫的威胁。2016年正式发布的花生基因组序列，可能有助于解释该物种耐旱性的分子机制。我们在此报告花生的基因表达谱分析，以全面了解其抗旱性。利用全转录组测序，我们分析了抗旱花生品种J11在干旱胁迫下的差异基因表达。将在6、12、18、24和48小时获得的混合样本与0小时的对照样本进行比较。总共发现了51554个基因，包括49289个已知基因和2265个未知基因。我们鉴定出224个差异表达的转录因子、296335个单核苷酸多态性（SNP）和28391个插入缺失（InDEL）。此外，我们检测到处理组和对照组基因表达谱的显著差异。比较两组后，鉴定出4648个基因。对数据的深入分析表明，大量基因与干旱胁迫相关，包括转录因子以及参与光合天线蛋白、碳代谢和柠檬酸循环的基因。本研究结果为花生成功建立抗旱性的多种机制提供了见解，从而有助于鉴定花生中与干旱管理相关的重要基因。基于RNA测序的转录组分析是该物种基因发现和分子标记开发的有力方法。
