两种花生（Arachis hypogaea L.）品种抗旱相关基因的比较转录组分析。

Comparative transcriptome analysis of genes involved in the drought stress response of two peanut (Arachis hypogaea L.) varieties.

机构信息

College of Agriculture, Shenyang Agricultural University, Shenyang, 110000, China.

College of Environment and Resources, Dalian Minzu University, Dalian, 116600, China.

出版信息

BMC Plant Biol. 2021 Jan 27;21(1):64. doi: 10.1186/s12870-020-02761-1.

DOI:10.1186/s12870-020-02761-1

PMID:33504328

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7839228/

Abstract

BACKGROUND

The peanut is one of the most important oil crops worldwide. Qualities and yields of peanut can be dramatically diminished by abiotic stresses particularly by drought. Therefore, it would be beneficial to gain a comprehensive understanding on peanut drought-responsive transcriptional regulatory activities, and hopefully to extract critical drought-tolerance-related molecular mechanism from it.

RESULTS

In this study, two peanut Arachis hypogaea L. varieties, NH5 (tolerant) and FH18 (sensitive), which show significantly differential drought tolerance, were screened from 23 main commercial peanut cultivars and used for physiological characterization and transcriptomic analysis. NH5 leaves showed higher water and GSH contents, faster stomatal closure, and lower relative conductivity (REC) than FH18. Under the time-course of drought-treatments 0 h (CK), 4 h (DT1), 8 h (DT2) and 24 h (DT3), the number of down-regulated differential expressed genes (DEGs) increased with the progression of treatments indicating repressive impacts on transcriptomes by drought in both peanut varieties.

CONCLUSIONS

Nevertheless, NH5 maintained more stable transcriptomic dynamics than FH18. Furthermore, annotations of identified DEGs implicate signal transduction, the elimination of reactive oxygen species, and the maintenance of cell osmotic potential which are key drought-tolerance-related pathways. Finally, evidences from the examination of ABA and SA components suggested that the fast stomatal closure in NH5 was likely mediated through SA rather than ABA signaling. In all, these results have provided us a comprehensive overview of peanut drought-responsive transcriptomic changes, which could serve as solid foundation for further identification of the molecular drought-tolerance mechanism in peanut and other oil crops.

摘要

背景

花生是全球最重要的油料作物之一。非生物胁迫，特别是干旱，会显著降低花生的品质和产量。因此，全面了解花生对干旱的转录调控活性，并从中提取关键的耐旱相关分子机制将是有益的。

结果

本研究从 23 个主要商业花生品种中筛选出两种花生 Arachis hypogaea L. 品种，NH5（耐旱）和 FH18（敏感），它们表现出明显的耐旱差异，用于生理特性和转录组分析。NH5 叶片的水分和 GSH 含量较高，气孔关闭较快，相对电导率（REC）较低。在干旱处理的时间过程中 0 h（CK）、4 h（DT1）、8 h（DT2）和 24 h（DT3），下调差异表达基因（DEGs）的数量随着处理的进展而增加，表明干旱对两种花生品种的转录组都有抑制作用。

结论

然而，NH5 保持了比 FH18 更稳定的转录组动态。此外，鉴定的 DEGs 的注释表明，信号转导、活性氧的消除以及细胞渗透势的维持是与耐旱相关的关键途径。最后，ABA 和 SA 成分的检测结果表明，NH5 中快速的气孔关闭可能是通过 SA 而不是 ABA 信号介导的。总之，这些结果为我们全面了解花生对干旱的转录组变化提供了基础，可为进一步鉴定花生和其他油料作物的分子耐旱机制提供依据。