Florida Institute of Technology, Melbourne, FL, USA.
University of Central Florida, Orlando, FL, USA.
Ultrason Sonochem. 2021 Apr;72:105427. doi: 10.1016/j.ultsonch.2020.105427. Epub 2020 Dec 26.
Carbon monoxide (CO), an endogenously produced gasotransmitter, has shown various therapeutic effects in previous studies. In this work, we developed an ultrasound responsive micelle for localized CO delivery. The micelle is composed of a pluronic shell and a core of a CO releasing molecule, CORM-2. The mechanism is based on the ultrasound response of pluronics, and the reaction between CORM-2 and certain biomolecules, e.g. cysteine. The latter allows CO release without significantly breaking the micelles. In a 3.5 mM cysteine solution, the micelles released low level of CO, indicating effective encapsulation of CORM-2. Treatment with a low intensity, non-focused ultrasound led to four times as much CO as the sample without ultrasonication, which is close to that of unencapsulated CORM-2. Significantly reduced proliferation of prostate cancer cells (PC-3) was observed 24 h after the PC-3 cells were treated with the CORM-2 micelles followed by ultrasound activation.
一氧化碳（CO）是一种内源性气体递质，先前的研究表明其具有多种治疗效果。在这项工作中，我们开发了一种超声响应胶束用于局部 CO 递送。该胶束由普朗尼克外壳和 CO 释放分子 CORM-2 的核组成。其机制基于普朗尼克的超声响应以及 CORM-2 与某些生物分子（例如半胱氨酸）之间的反应。后者允许 CO 释放，而不会显著破坏胶束。在 3.5mM 半胱氨酸溶液中，胶束释放出低水平的 CO，表明 CORM-2 得到了有效包封。用低强度、非聚焦超声处理后，CO 的释放量是未超声处理样品的四倍，接近于未包封的 CORM-2。经超声激活后，用 CORM-2 胶束处理 PC-3 细胞 24 小时后，显著抑制了前列腺癌细胞（PC-3）的增殖。
