Park Slmaro, Jung Han-Sung, Jung Young-Soo, Nam Woong, Cha Jung Yul, Jung Hwi-Dong
Department of Oral & Maxillofacial Surgery, College of Dentistry, Yonsei University, 50-1 Yonsei-Ro, Seodeamun-Gu, Seoul 03722, Korea.
Division in Anatomy and Developmental Biology, Department of Oral Biology, Oral Science Research Center, BK21 PLUS Project, Yonsei University College of Dentistry, Seoul 03722, Korea.
J Clin Med. 2020 Dec 24;10(1):30. doi: 10.3390/jcm10010030.
Decompression followed by enucleation, which is one of the treatments used for odontogenic keratocysts (OKCs), is frequently used in OKC lesions of large sizes. This method offers the advantage of minimizing the possibility of sensory impairment without creating a wide-range bone defect; moreover, the recurrence rate can be significantly lower than following simple enucleation. This study aimed to assess the changes in histology and expression of proliferation markers in OKCs before and after decompression treatment. A total of 38 OKC tissue samples from 19 patients who had undergone decompression therapy were examined morphologically and immunohistochemically to observe changes in proliferative activity before and after decompression. The markers used for immunohistochemistry (IHC) staining were Bcl-2, epidermal growth factor receptor (EGFR), Ki-67, P53, PCNA, and SMO. The immunohistochemistry positivity of the 6 markers was scored by using software ImageJ, version 1.49, by quantifying the intensity and internal density of IHC-stained epithelium. The values of Bcl-2, Ki-67, P53, proliferating cell nuclear antigen (PCNA), and SMO in OKCs before and after decompression showed no significant change. No correlation between clinical shrinkage and morphologic changes or expression of proliferation and growth markers could be found. There was no statistical evidence that decompression treatment reduces potentially aggressive behavior of OKC within the epithelial cyst lining itself. This might indicate that decompression does not change the biological behavior of the epithelial cyst lining or the recurrence rate.
减压后摘除术是用于治疗牙源性角化囊肿（OKC）的方法之一，常用于较大尺寸的OKC病变。该方法的优点是可将感觉障碍的可能性降至最低，且不会造成大范围的骨缺损；此外，其复发率可能显著低于单纯摘除术。本研究旨在评估减压治疗前后OKC的组织学变化以及增殖标志物的表达情况。对19例接受减压治疗的患者的38份OKC组织样本进行了形态学和免疫组织化学检查，以观察减压前后增殖活性的变化。用于免疫组织化学（IHC）染色的标志物为Bcl-2、表皮生长因子受体（EGFR）、Ki-67、P53、增殖细胞核抗原（PCNA）和SMO。使用ImageJ 1.49软件通过量化IHC染色上皮细胞的强度和内部密度，对这6种标志物的免疫组织化学阳性进行评分。减压前后OKC中Bcl-2、Ki-67、P53、增殖细胞核抗原（PCNA）和SMO的值无显著变化。未发现临床缩小与形态学变化或增殖及生长标志物表达之间存在相关性。没有统计学证据表明减压治疗可降低上皮囊肿衬里内OKC的潜在侵袭性行为。这可能表明减压不会改变上皮囊肿衬里的生物学行为或复发率。
