Noguchi Hirofumi
Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, Japan.
J Clin Med. 2020 Dec 23;10(1):10. doi: 10.3390/jcm10010010.
The islet purification step in clinical islet isolation is important for minimizing the risks associated with intraportal infusion. Continuous density gradient with a COBE 2991 cell processor is commonly used for clinical islet purification. However, the high shear force involved in the purification method using the COBE 2991 cell processor causes mechanical damage to the islets. We and other groups have shown human/porcine islet purification using large cylindrical plastic bottles. Shear stress can be minimized or eliminated using large cylindrical plastic bottles because the bottles do not have a narrow segment and no centrifugation is required during tissue loading and the collection processes of islet purification. This review describes current advances in islet purification from large mammals and humans using a COBE 2991 cell processor versus large cylindrical plastic bottles.
临床胰岛分离中的胰岛纯化步骤对于将门静脉内输注相关风险降至最低至关重要。使用COBE 2991细胞处理器进行连续密度梯度分离通常用于临床胰岛纯化。然而，使用COBE 2991细胞处理器的纯化方法中涉及的高剪切力会对胰岛造成机械损伤。我们和其他研究小组已展示了使用大型圆柱形塑料瓶进行人/猪胰岛纯化。使用大型圆柱形塑料瓶可将剪切应力降至最低或消除，因为瓶子没有狭窄部分，并且在胰岛纯化的组织加载和收集过程中无需离心。本综述描述了使用COBE 2991细胞处理器与大型圆柱形塑料瓶从大型哺乳动物和人类中纯化胰岛的当前进展。
