Yoon Soyon, Song Seokcheon, Shin Jae Woo, Kang Sini, Kim Hye Young, You Hyun Ju
Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul 08826, Korea.
Laboratory of Mucosal Immunology, Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 110799, Korea.
Antioxidants (Basel). 2020 Dec 23;10(1):6. doi: 10.3390/antiox10010006.
The increasing prevalence of allergic asthma has become the world's major health issue. Current treatments for allergic asthma focus on treating symptoms, while permanent cures still remain undiscovered. In this study, we investigated the effect of Korean traditional herbal remedy, Pyunkang-tang (PGT)-composed of six plants-on asthma alleviation in a mouse model. The PGT mixture was orally gavaged to mice (PM group, 20 mg/mouse/day) from 7 days before sensitization with ovalbumin (OVA) (day -7). On day 0 and day 14, mice from OVA-control ( = 9) and PM group ( = 8) were sensitized with OVA and alum through intraperitoneal injection. On days 18~20, OVA was challenged to mice through nasal injection and sacrificed next day. Cell profile in lung tissue was analyzed by flow cytometry and RT-qPCR analysis, and the number of eosinophils and expression of were significantly reduced in the PM group. Lung tissue was examined with hematoxylin and eosin (H&E) and Alcian blue/periodic acid-Schiff (AB-PAS) staining. Noticeably reduced eosinophil infiltration around bronchioles was displayed in the PM group compared to the OVA-control group. Furthermore, PGT-treated mice showed a significant reduction in IL-13 and a mild reduction in IL-5 in lungs. A decreasing tendency of IL-5/13 (+) CD4+ T cells and IL-13(+) innate lymphoid cells (ILCs) and a significant reduction in IL5(+) ILCs were also observed. When treating PGT on murine lung epithelial cells stimulated by papain, there was a significant reduction in IL-33 mRNA expression levels. Taken together, oral delivery of PGT successfully alleviated asthmatic responses provoked by OVA in a mouse model and could lead to novel therapies for allergic asthma.
过敏性哮喘患病率的不断上升已成为全球主要的健康问题。目前过敏性哮喘的治疗主要集中在症状治疗上，而永久性治愈方法仍未找到。在本研究中，我们调查了由六种植物组成的韩国传统草药制剂平康汤（PGT）对小鼠模型哮喘缓解的影响。从用卵清蛋白（OVA）致敏前7天（第-7天）开始，对小鼠进行PGT混合物灌胃（PM组，20mg/小鼠/天）。在第0天和第14天，OVA对照组（n = 9）和PM组（n = 8）的小鼠通过腹腔注射OVA和明矾进行致敏。在第18至20天，通过鼻内注射对小鼠进行OVA激发，并于次日处死。通过流式细胞术和RT-qPCR分析对肺组织中的细胞谱进行分析，结果显示PM组中嗜酸性粒细胞数量和IL-5表达显著降低。用苏木精和伊红（H&E）以及阿尔辛蓝/过碘酸希夫（AB-PAS）染色对肺组织进行检查。与OVA对照组相比，PM组中细支气管周围嗜酸性粒细胞浸润明显减少。此外，PGT处理的小鼠肺中IL-13显著降低，IL-5轻度降低。还观察到IL-5/13(+) CD4+ T细胞和IL-13(+)天然淋巴细胞（ILC）有下降趋势，IL5(+) ILC显著减少。在用木瓜蛋白酶刺激的小鼠肺上皮细胞上处理PGT时，IL-33 mRNA表达水平显著降低。综上所述，口服PGT成功减轻了小鼠模型中OVA诱发的哮喘反应，并可能为过敏性哮喘带来新的治疗方法。
