Zvikas Vaidotas, Urbanaviciute Ieva, Bernotiene Rasa, Kulakauskiene Deimante, Morkunaite Urte, Balion Zbigniev, Majiene Daiva, Liaudanskas Mindaugas, Viskelis Pranas, Jekabsone Aiste, Jakstas Valdas
Institute of Pharmaceutical Technologies, Lithuanian University of Health Sciences, Sukilėlių av. 13, LT-50162 Kaunas, Lithuania.
Laboratory of Biochemistry and Technology, Institute for Horticulture, Lithuanian Research Centre for Agriculture and Forestry, Kauno str. 30, LT-54333 Babtai, Lithuania.
Foods. 2020 Dec 23;10(1):18. doi: 10.3390/foods10010018.
Glioblastoma multiforme is an aggressive and invasive disease with no efficient therapy available, and there is a great need for finding alternative treatment strategies. This study aimed to investigate anticancer activity of the extracts of the Japanese quince (JQ) cultivars 'Darius', 'Rondo', and 'Rasa' leaf extracts on glioblastoma C6 and HROG36 cells. As identified by ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry, the extracts contained three prevailing groups of phenols: hydroxycinnamic acid derivatives; flavan-3-ols; and flavonols. Sixteen phenols were detected; the predominant compound was chlorogenic acid. The sum of detected phenols varied significantly between the cultivars ranging from 9322 µg/g ('Rondo') to 17,048 µg/g DW ('Darius'). Incubation with the extracts decreased the viability of glioblastoma HROG36 cells with an efficiency similar to temozolomide, a drug used for glioblastoma treatment. In the case of C6 glioblastoma cells, the extracts were even more efficient than temozolomide. Interestingly, primary cerebellar neuronal-glial cells were significantly less sensitive to the extracts compared to the cancer cell lines. The results showed that JQ leaf ethanol extracts are rich in phenolic compounds, can efficiently reduce glioblastoma cell viability while preserving non-cancerous cells, and are worth further investigations as potential anticancer drugs.
多形性胶质母细胞瘤是一种侵袭性疾病，目前尚无有效的治疗方法，因此迫切需要寻找替代治疗策略。本研究旨在探讨日本榅桲（JQ）品种‘大流士’、‘朗多’和‘拉萨’叶片提取物对胶质母细胞瘤C6和HROG36细胞的抗癌活性。通过超高效液相色谱电喷雾电离串联质谱鉴定，提取物中含有三类主要酚类物质：羟基肉桂酸衍生物、黄烷-3-醇和黄酮醇。共检测到16种酚类物质，其中主要化合物为绿原酸。不同品种间检测到的酚类物质总量差异显著，范围从9322 µg/g（‘朗多’）到17048 µg/g干重（‘大流士’）。提取物处理后，胶质母细胞瘤HROG36细胞的活力降低，其效果与用于治疗胶质母细胞瘤的药物替莫唑胺相似。对于C6胶质母细胞瘤细胞，提取物的效果甚至比替莫唑胺更好。有趣的是，与癌细胞系相比，原代小脑神经元-胶质细胞对提取物的敏感性明显较低。结果表明，JQ叶片乙醇提取物富含酚类化合物，能够有效降低胶质母细胞瘤细胞的活力，同时保护非癌细胞，作为潜在的抗癌药物值得进一步研究。
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