School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Xianlin Road #138, Nanjing 210023, China.
Shanghai Institue of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
Molecules. 2020 Dec 23;26(1):44. doi: 10.3390/molecules26010044.
Twelve compounds, including two new aristolochic acid analogues with a formyloxy moiety (9-10) and 10 known aristolochic acid derivates (1-8 and 11-12), were obtained from the roots of . Their structures were elucidated using extensive spectroscopic methods. Their cytotoxic activity in human proximal tubular cells HK-2 was evaluated by the MTT method, which has been widely used to assess cell viability. Among these molecules, compounds and were found to be more cytotoxic. Furthermore, molecular modeling was used to evaluate, for the first time, the interactions of compounds and with the target protein organic anionic transporter 1 (OAT1) that plays a key role in mediating aristolochic acid nephropathy. Structure-activity relationships are briefly discussed.
从 中分离得到了 12 种化合物，包括两种具有甲氧基部分的新马兜铃酸类似物（9-10）和 10 种已知的马兜铃酸衍生物（1-8 和 11-12）。采用多种光谱方法阐明了它们的结构。采用 MTT 法评估了这些分子在人近端肾小管细胞 HK-2 中的细胞毒性活性，MTT 法已广泛用于评估细胞活力。在这些分子中，发现化合物 和 具有更强的细胞毒性。此外，首次采用分子建模评估了化合物 和 与靶蛋白有机阴离子转运蛋白 1（OAT1）的相互作用，OAT1 在介导马兜铃酸肾病中起关键作用。简要讨论了构效关系。
