Křížkovská Bára, Kumar Rohitesh, Řehořová Kateřina, Sýkora David, Dobiasová Simona, Kučerová Denisa, Tan Maria Carmen, Linis Virgilio, Oyong Glenn, Ruml Tomáš, Lipov Jan, Viktorová Jitka
Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic.
Department of Analytical Chemistry, University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic.
Pharmaceuticals (Basel). 2020 Dec 26;14(1):16. doi: 10.3390/ph14010016.
P. Beauv. is a group of vascular plants in the family Selaginellaceae Willk., found worldwide and numbering more than 700 species, with some used as foods and medicines. The aim of this paper was to compare methanolic (MeOH) and dichloromethane (DCM) extracts of eight species on the basis of their composition and biological activities. Six of these species are underinvestigated. Using ultra-high performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) analysis, we identified a total of 193 compounds among the tested species, with flavonoids predominating. MeOH extracts recovered more constituents that were detected, including selaginellins, the occurrence of which is only typical for this plant genus. Of all the tested species, contained the highest number of identified selaginellins. The majority of the compounds were identified in , the fewest compounds in . All the tested species demonstrated antioxidant activity using oxygen radical absorption capacity (ORAC) assay, which showed that MeOH extracts had higher antioxidant capacity, with the half maximal effective concentration (EC) ranging from 12 ± 1 ( ) to 124 ± 2 ( ) mg/L. The antioxidant capacity was presumed to be correlated with the content of flavonoids, (neo)lignans, and selaginellins. Inhibition of acetylcholinesterase (AChE) was mostly discerned in DCM extracts and was only exhibited in , , , and extracts with the half maximal inhibitory concentration (IC) in the range of 19 ± 3 to 62 ± 1 mg/L. Substantial cytotoxicity against cancer cell lines was demonstrated by the MeOH extract of , where the ratio of the IC HEK (human embryonic kidney) to IC HepG2 (hepatocellular carcinoma) was 7.9 ± 0.2. MeOH extracts inhibited the production of nitrate oxide and cytokines in a dose-dependent manner. Notably, halved the production of NO, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 at the following concentrations: 105 ± 9, 11 ± 1, and 10 ± 1 mg/L, respectively. Our data confirmed that extracts from species exhibited cytotoxicity against cancer cell lines and AChE inhibition. The activity observed in was the most promising and is worth further exploration.
卷柏属是卷柏科（Willk.）中的一组维管植物，在全球范围内均有发现，有700多种，其中一些可作为食物和药物。本文的目的是基于八种植物的甲醇提取物（MeOH）和二氯甲烷提取物（DCM）的成分和生物活性进行比较。其中六种植物尚未得到充分研究。通过超高效液相色谱-高分辨率质谱（UHPLC-HRMS）分析，我们在受试植物中总共鉴定出193种化合物，其中黄酮类化合物占主导。甲醇提取物中检测到的成分更多，包括卷柏素，其仅在该植物属中出现。在所有受试植物中，[具体植物名称1]所含已鉴定的卷柏素数量最多。大多数化合物在[具体植物名称2]中被鉴定出来，在[具体植物名称3]中鉴定出的化合物最少。使用氧自由基吸收能力（ORAC）测定法，所有受试植物均表现出抗氧化活性，结果表明甲醇提取物具有更高的抗氧化能力，半数最大有效浓度（EC）范围为12±1（[植物名称4]）至124±2（[植物名称5]）mg/L。推测抗氧化能力与黄酮类化合物、（新）木脂素和卷柏素的含量相关。乙酰胆碱酯酶（AChE）的抑制作用主要在二氯甲烷提取物中观察到，并且仅在[具体植物名称6]、[具体植物名称7]、[具体植物名称8]和[具体植物名称9]的提取物中表现出来，半数最大抑制浓度（IC）在19±3至62±1mg/L范围内。[具体植物名称10]的甲醇提取物对癌细胞系表现出显著的细胞毒性，其ICHEK（人胚肾细胞）与IC HepG2（肝癌细胞）的比值为7.9±0.2。甲醇提取物以剂量依赖的方式抑制一氧化氮和细胞因子的产生。值得注意的是，[具体植物名称11]在以下浓度下分别使一氧化氮、肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6的产生减半：105±9、11±1和10±x mg/L。我们的数据证实，[具体植物名称12]的提取物对癌细胞系表现出细胞毒性并具有AChE抑制作用。在[具体植物名称13]中观察到的活性最具前景，值得进一步探索。
需注意，原文中部分植物名称处标注了[具体植物名称x]，这是因为原文此处未给出具体植物名称，翻译时保留原样以提醒读者此处信息缺失。你可根据实际情况补充完整相关植物名称信息。
