Arlyapov V A, Kharkova A S, Kurbanaliyeva S K, Kuznetsova L S, Machulin A V, Tarasov S E, Melnikov P V, Ponamoreva O N, Alferov V A, Reshetilov A N
Tula State University, 92 Lenin Pr., Tula, 300012, Russia.
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, prosp. Nauki 5, Pushchino, Moscow Region, 142290, Russia.
Enzyme Microb Technol. 2021 Feb;143:109706. doi: 10.1016/j.enzmictec.2020.109706. Epub 2020 Nov 16.
This work investigated the use of redox-active polymers based on bovine serum albumin and chitosan, covalently bound to mediators neutral red and ferrocene and containing carbon nanotubes, for immobilization of Paracoccus yeei VKM B-3302 bacteria. The structures of produced polymers were studied by IR spectroscopy and scanning electron microscopy. Cyclic voltammetry and impedance spectroscopy found the electrochemical characteristics of the investigated systems: the heterogeneous electron transfer rate constant, the constant of the rate of interaction with P. yeei bacteria and the impedance. The systems containing carbon nanotubes and ferrocene-based redox-active polymer proved to be the most promising. Biosensors formed using the hybrid polymers had a high sensitivity with the lower boundary of 0.1 mg/dm of the detected BOD concentrations and a high correlation (R = 0.9916) with the standard BOD assay of surface water samples.
本研究探讨了基于牛血清白蛋白和壳聚糖的氧化还原活性聚合物的用途，这些聚合物与中性红和二茂铁等介质共价结合并含有碳纳米管，用于固定化耶氏副球菌VKM B-3302细菌。通过红外光谱和扫描电子显微镜研究了所制备聚合物的结构。循环伏安法和阻抗谱测定了所研究体系的电化学特性：异相电子转移速率常数、与耶氏副球菌相互作用的速率常数以及阻抗。含有碳纳米管和基于二茂铁的氧化还原活性聚合物的体系被证明是最有前景的。使用杂化聚合物形成的生物传感器具有高灵敏度，检测到的生化需氧量浓度下限为0.1mg/dm，并且与地表水样品的标准生化需氧量测定具有高度相关性（R = 0.9916）。
