Sairi Anith M M, Ismail Siti Izera, Sukor Arina, Rashid Noraswati M N, Saad Norsazilawati, Jamian Syari, Abdullah Sumaiyah
Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Evid Based Complement Alternat Med. 2020 Dec 9;2020:7415672. doi: 10.1155/2020/7415672. eCollection 2020.
Polypores are mushrooms which are rich in bioactivities and for generations, they have been widely used as herbal remedies. Despite their significant importance in treatments of various health issues, only a few local species have been reported for their pharmacological potentials. The present study was carried out to establish cytotoxicity potentials of , a local polypore species collected from forested areas in Malaysia at cellular levels on normal human lung (MRC5) and human lung carcinoma (A549) cell lines. Survival and inhibition rates were analyzed by 3-(4, 5)-dimethylthiahiazo (-z-y-l)-2,5-diphenyltetrazoliumbromide (MTT) while monitoring changes on cellular shapes by inverted phase contrast microscopy. Survival rates of MRC5 cells were observed to be significantly higher than A549 after treatments with various concentrations of polypore extracts. MRC5 cells showed excellence in survival performance when treated with hot and cold aqueous extracts. Cold aqueous extract showed higher cytotoxicity activities compared to hot aqueous extract ( < 0.0001) with inhibitory concentration (IC) values of 414.29 g/ml and >1000 g/ml, respectively. Treatments with tamoxifen as a control exhibited necrotic features in both cell lines. The results suggest that possesses pharmacological potentials that can be utilized for human consumption as a new bioresource alternative, thus encouraging research advancement in mycological and nutraceutical studies.
多孔菌是一类富含生物活性的蘑菇，长期以来，它们一直被广泛用作草药。尽管它们在治疗各种健康问题方面具有重要意义，但只有少数本地物种的药理潜力得到了报道。本研究旨在确定从马来西亚森林地区采集的一种本地多孔菌在细胞水平上对正常人肺（MRC5）和人肺癌（A549）细胞系的细胞毒性潜力。通过3-（4,5）-二甲基噻唑-2,5-二苯基四氮唑溴盐（MTT）分析细胞存活率和抑制率，同时通过倒置相差显微镜监测细胞形态的变化。在用不同浓度的多孔菌提取物处理后，观察到MRC5细胞的存活率明显高于A549细胞。用热和冷的水提取物处理时，MRC5细胞表现出优异的存活性能。与热的水提取物相比，冷的水提取物显示出更高的细胞毒性活性（P<0.0001），其抑制浓度（IC）值分别为414.29μg/ml和>1000μg/ml。用他莫昔芬作为对照进行处理时，两种细胞系均表现出坏死特征。结果表明，该多孔菌具有可作为新的生物资源替代品供人类食用的药理潜力，从而促进了真菌学和营养保健品研究的进展。
