Zhong Mengya, Chen Zhijian, Yan Yang, Bahet Argen, Cai Xin, Chen Huiyu, Ran Honggang, Qu Kaiyong, Han Zhaopu, Zhuang Guohong, Zhang Shifeng, Wang Yinan
Cancer Research Center, School of Medicine, Xiamen University, Xiamen, Fujian 361005, P.R. China.
Department of Gastrointestinal Surgery, Zhongshan Hospital of Xiamen University, Xiamen, Fujian 361004, P.R. China.
Oncol Lett. 2021 Feb;21(2):118. doi: 10.3892/ol.2020.12379. Epub 2020 Dec 15.
The tumor necrosis factor α-induced protein 8 (TNFAIP8)-like (TIPE) protein family comprises four members, namely TNFAIP8, TIPE1, TIPE2 and TIPE3, which are involved in multiple processes in cancer. The current study aimed to investigate the expression patterns and potential clinical roles of the TIPE family members in human colorectal cancer (CRC). Paired tumor and adjacent tissue samples were collected from 49 patients with CRC, and the relative mRNA expression levels of the TIPE family members in these samples were evaluated by using reverse transcription-quantitative PCR, and the protein levels in five randomly selected pairs of tumor and adjacent tissue samples were detected by western blot analysis. The mRNA expression levels of the TIPE family members were significantly downregulated in CRC tumor tissues compared with those in the adjacent tissues; however, within each sample, TNFAIP8 and TIPE3 protein levels were only partially consistent with their mRNA levels. In addition, the mRNA expression levels between each pair of TIPE family members exhibited a positive linear relationship, and TIPE2 mRNA levels exhibited strong linear associations with those of TNFAIP8 and TIPE1. TNFAIP8 mRNA expression levels in tumor tissues were significantly associated with the tumor differentiation grade, and TIPE2 mRNA expression levels in tumor tissues were significantly associated with sex. TIPE1 and TIPE3 mRNA expression levels in tumor tissues exhibited no associations with patient clinicopathological characteristics. In addition, the mRNA expression patterns of the TIPE family members were analyzed using data from The Cancer Genome Atlas data set, and the results also demonstrated that TNFAIP8, TIPE2 and TIPE3 mRNA levels were downregulated in patients with colon adenocarcinoma compared with those in normal controls. These results provided evidence that the four members of the TIPE family may affect each other to mediate the carcinogenesis of CRC, and that TIPE2 may serve an important role in CRC.
肿瘤坏死因子α诱导蛋白8（TNFAIP8）样（TIPE）蛋白家族由四个成员组成，即TNFAIP8、TIPE1、TIPE2和TIPE3，它们参与癌症的多个过程。本研究旨在探讨TIPE家族成员在人类结直肠癌（CRC）中的表达模式及潜在临床作用。收集了49例CRC患者的肿瘤组织及癌旁组织样本，采用逆转录定量PCR评估这些样本中TIPE家族成员的相对mRNA表达水平，并通过蛋白质印迹分析检测了随机选取的5对肿瘤组织和癌旁组织样本中的蛋白水平。与癌旁组织相比，CRC肿瘤组织中TIPE家族成员的mRNA表达水平显著下调；然而，在每个样本中，TNFAIP8和TIPE3的蛋白水平仅部分与其mRNA水平一致。此外，每对TIPE家族成员之间的mRNA表达水平呈正线性关系，且TIPE2的mRNA水平与TNFAIP8和TIPE1的mRNA水平呈强线性关联。肿瘤组织中TNFAIP8的mRNA表达水平与肿瘤分化程度显著相关，肿瘤组织中TIPE2的mRNA表达水平与性别显著相关。肿瘤组织中TIPE1和TIPE3的mRNA表达水平与患者临床病理特征无相关性。此外，利用癌症基因组图谱数据集的数据对TIPE家族成员的mRNA表达模式进行了分析，结果还表明，与正常对照相比，结肠腺癌患者中TNFAIP8、TIPE2和TIPE3的mRNA水平下调。这些结果提供了证据，表明TIPE家族的四个成员可能相互影响以介导CRC的致癌作用，且TIPE2可能在CRC中发挥重要作用。
