Tan Qiang, Shi Shuang, Liang Jingjie, Cao Dingren, Wang Shaoyu, Wang Zhengguang
College of Animal Science, Zhejiang University, Hangzhou 310058, PR China.
Hainan Institute of Zhejiang University, Sanya 572000, PR China.
Mol Ther Nucleic Acids. 2020 Nov 5;23:217-231. doi: 10.1016/j.omtn.2020.10.043. eCollection 2021 Mar 5.
Communication between maternal uterus and blastocyst occurs in the early stages of pregnancy, and the interaction influences the success of embryo implantation. Whereas small extracellular vesicles (sEVs) play an essential role in mediating intercellular communication in numerous biological processes, their role in embryo implantation during the window of implantation (WOI) remains poorly defined. Here, we report that endometrial epithelial cells (EECs) secrete sEVs during early pregnancy, which affects the trophoblast behaviors (migration, invasion, and proliferation), thus influencing embryo implantation. We show that microRNA (miR)-100-5p, sEVs containing microRNA (miRNA), activates both focal adhesion kinase (FAK) and c-Jun N-terminal kinase (JNK), as well as contributes to trophoblast migration and invasion. Furthermore, our findings indicate that the sEV miR-100-5p promotes angiogenesis during the implantation process. In conclusion, this study reveals a novel mechanism by which EEC-derived sEV miR-100-5p crosstalks with trophoblasts, leading to an enhanced ability for implantation.
母体子宫与囊胚之间的通讯发生在妊娠早期，这种相互作用影响胚胎着床的成功率。尽管小细胞外囊泡（sEVs）在众多生物过程中介导细胞间通讯中发挥着重要作用，但其在着床窗（WOI）期间胚胎着床中的作用仍不清楚。在此，我们报道子宫内膜上皮细胞（EECs）在妊娠早期分泌sEVs，其影响滋养层细胞行为（迁移、侵袭和增殖），从而影响胚胎着床。我们发现，微小RNA（miR）-100-5p，即包含微小RNA（miRNA）的sEVs，激活黏着斑激酶（FAK）和c-Jun氨基末端激酶（JNK），并促进滋养层细胞迁移和侵袭。此外，我们的研究结果表明，sEV miR-100-5p在着床过程中促进血管生成。总之，本研究揭示了一种新机制，即EEC来源的sEV miR-100-5p与滋养层细胞相互作用，从而提高着床能力。
