Department of Pathology, University of Michigan Medical School, Ann Arbor, MI 48109, USA.
STAR Protoc. 2020 Oct 20;1(3):100136. doi: 10.1016/j.xpro.2020.100136. eCollection 2020 Dec 18.
Embryonic diapause is a naturally occurring strategy in mammals that determines successful rates of gestation under unfavorable conditions. This dormant state can be captured in the form of quiescent mouse embryonic stem cells (ESCs). Here, we present a step-by-step protocol to derive quiescent ESCs that naturally exist in culture by harnessing the heterogeneity of mitochondrial activity. The derived quiescent ESCs with low mitochondrial activity can be utilized as a surrogate to study stages of early embryonic development. For complete details on the use and execution of this protocol, please refer to Khoa et al. (2020).
胚胎休眠是哺乳动物中一种自然发生的策略，可确定在不利条件下妊娠的成功率。这种休眠状态可以以静止的小鼠胚胎干细胞（ESCs）的形式捕获。在这里，我们通过利用线粒体活性的异质性，提出了一种从培养中自然存在的静止 ESC 中获得的分步方案。具有低线粒体活性的衍生静止 ESC 可用于研究早期胚胎发育阶段。有关使用和执行此方案的完整详细信息，请参阅 Khoa 等人。（2020 年）。
