获取人乳腺脂肪细胞用于乳腺癌细胞共培养研究。

Department of Human Anatomy and Embryology, Faculty of Medicine; Biopathology and Medicine Regenerative Institute (IBIMER); Instituto de Investigación Biosanitaria de Granada (ibs.GRANADA); and Excellence Research Unit "Modeling Nature" (MNat), University of Granada, Granada 18071, Spain.

Breast Cancer Program, Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University, Washington, DC 20007, USA.

STAR Protoc. 2020 Dec 11;1(3):100197. doi: 10.1016/j.xpro.2020.100197. eCollection 2020 Dec 18.

Primary human breast cancers invade surrounding fat and contact adipocytes, inflammatory infiltrates, and fibrous stroma. This tissue niche influences breast tumor progression. Here, we present a protocol to enable the study of the complex interactions that occur between breast cancer cells and adipose cells. We describe how to obtain different adipose cell populations, including adipose-derived stem cells, immature adipocytes, and mature adipocytes, from human breast fat tissue and detail the application for co-culture assays with breast cancer cells. For complete details on the use and execution of this protocol, please refer to Picon-Ruiz et al. (2016) and Qureshi et al. (2020).

原发性人乳腺癌侵犯周围脂肪组织并与脂肪细胞、炎症浸润物和纤维基质接触。这个组织小生境影响着乳腺肿瘤的进展。在这里，我们提供了一个方案，以研究乳腺癌细胞和脂肪细胞之间发生的复杂相互作用。我们描述了如何从人乳腺脂肪组织中获得不同的脂肪细胞群体，包括脂肪来源的干细胞、未成熟脂肪细胞和成熟脂肪细胞，并详细说明了与乳腺癌细胞共培养实验的应用。有关该方案使用和执行的完整详细信息，请参见 Picon-Ruiz 等人（2016 年）和 Qureshi 等人（2020 年）的文献。