Shaanxi Key Laboratory of Brain Disorders, Institute of Basic Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi 710021, China.
Center for Ecological and Environmental Sciences, Northwestern Polytechnical University, Xi'an, Shaanxi 710072, China.
Zool Res. 2021 Jan 18;42(1):130-134. doi: 10.24272/j.issn.2095-8137.2020.258.
The Atlantic sea nettle ( ) has an important evolutionary position due to its high ecological value. However, due to limited sequencing technologies and complex jellyfish genomic sequences, the current genome assembly is highly fragmented. Here, we used the most advanced high-throughput chromosome conformation capture (Hi-C) technology to obtain high-coverage sequencing data of the genome. We then anchored these data to the previously published contig-level assembly to improve the genome. Finally, a high-continuity genome sequence of was successfully assembled, which contained 1 882 scaffolds with a N50 length of 3.83 Mb. The N50 length of the genome assembly was 5.23 times longer than the previously released one, and additional analysis revealed that it had a high degree of genomic continuity and accuracy. Acquisition of the high-continuity genome sequence of not only provides a basis for the study of jellyfish evolution through comparative genomics but also provides an important resource for studies on jellyfish growth and development.
大西洋海荨麻（ ）具有重要的进化地位，因为它具有很高的生态价值。然而，由于测序技术的限制和复杂的水母基因组序列，目前的 基因组组装高度碎片化。在这里，我们使用最先进的高通量染色体构象捕获（Hi-C）技术获得了 基因组的高覆盖测序数据。然后，我们将这些数据锚定到之前发布的基于 contig 的组装上，以改进基因组。最后，成功组装了一个具有高连续性的 基因组序列，其中包含 1882 个支架，N50 长度为 3.83Mb。该基因组组装的 N50 长度比之前发布的长 5.23 倍，额外的分析表明它具有高度的基因组连续性和准确性。获得大西洋海荨麻的高连续性基因组序列不仅为通过比较基因组学研究水母进化提供了基础，也为水母生长和发育的研究提供了重要资源。
