Defence Institute of Physiology & Allied Sciences (DIPAS), Defence R&D Organization (DRDO), Timarpur, New Delhi, 110054, India.
Defence Institute of Physiology & Allied Sciences (DIPAS), Defence R&D Organization (DRDO), Timarpur, New Delhi, 110054, India.
Biomed Pharmacother. 2021 Jan;133:111083. doi: 10.1016/j.biopha.2020.111083. Epub 2020 Dec 8.
Apo-A1 is correlated with conditions like hyperlipidemia, cardiovascular diseases, high altitude pulmonary edema and etc. where hypoxia constitutes an important facet.Hypoxia causes oxidative stress, vaso-destructive and inflammatory outcomes.Apo-A1 is reported to have vasoprotective, anti-oxidative, anti-apoptotic, and anti-inflammatory effects. However, effects of Apo-A1 augmentation during hypoxia exposure are unknown.In this study, we investigated the effects of exogenously supplementing Apo-A1-mimetic peptide on SD rats during hypoxia exposure. For easing the processes of delivery, absorption and bio-availability, Apo-A1 mimetic peptide D4F was used. The rats were given 10 mg/kg BW dose (i.p.) of D4F for 7 days and then exposed to hypoxia. D4F was observed to attenuate both oxidative stress and inflammation during hypoxic exposure. D4F improved energy homeostasis during hypoxic exposure. D4F did not affect HIF-1a levels during hypoxia but increased MnSOD levels while decreasing CRP and Apo-B levels. D4F showed promise as a prophylactic against hypoxia exposure.
载脂蛋白 A1 与高血脂症、心血管疾病、高原肺水肿等疾病有关，其中缺氧是一个重要方面。缺氧会导致氧化应激、血管破坏和炎症等后果。有报道称载脂蛋白 A1 具有血管保护、抗氧化、抗细胞凋亡和抗炎作用。然而，在缺氧暴露期间补充载脂蛋白 A1 的效果尚不清楚。在这项研究中，我们研究了在缺氧暴露期间外源性补充载脂蛋白 A1 模拟肽对 SD 大鼠的影响。为了便于给药、吸收和生物利用度，使用了载脂蛋白 A1 模拟肽 D4F。大鼠接受了 10mg/kg BW 剂量（ip）的 D4F 治疗 7 天，然后暴露于缺氧环境中。结果发现，D4F 可减轻缺氧暴露期间的氧化应激和炎症。D4F 改善了缺氧暴露期间的能量稳态。D4F 对缺氧期间的 HIF-1a 水平没有影响，但增加了 MnSOD 水平，同时降低了 CRP 和 Apo-B 水平。D4F 作为缺氧暴露的预防措施具有一定的潜力。
