Study on the Function and Mechanism of Lin28B in the Formation of Chicken Primordial Germ Cells.

and are two homologues of the same family of RNA binding proteins (RBPs). The function and molecular mechanism of in the formation of primordial germ cells (PGCs) are very clear, but the related research on is rarely reported. Here, we found that the overexpression of can promote the formation of PGC in vivo. Furthermore, the overexpression of also resulted in the inhibition of totipotency gene expression and upregulated the PGCs marker genes, and a significant increase in the number of PGCs in genital ridge, as detected by Periodic Acid-Schiff(PAS) staining. However, the inhibited expression showed completely opposite results, which were confirmed on the PGC induction model in vitro. Mechanistically, we found that the overexpression of can inhibit the maturation of , and the results of high-throughput sequencing indicated that was a negative regulator of the formation process of PGCs. Therefore, we conclude that our results determine that participates in the formation of PGCs through , which set a theoretical foundation for improving the function and mechanism of family in the formation of PGCs.