Opt Express. 2020 Nov 23;28(24):36643-36655. doi: 10.1364/OE.399713.
Despite the existence of various neural recording and mapping techniques, there is an open territory for the emergence of novel techniques. The current neural imaging and recording techniques suffer from invasiveness, a time-consuming labeling process, poor spatial/ temporal resolution, and noisy signals. Among others, neuroplasmonics is a label-free and nontoxic recording technique with no issue of photo-bleaching or signal-averaging. We introduced an integrated plasmonic-ellipsometry platform for membrane activity detection with cost-effective and high-quality grating extracted from commercial DVDs. With ellipsometry technique, one can measure both amplitude (intensity) and phase difference of reflected light simultaneously with high signal to noise ratio close to surface plasmon resonances, which leads to the enhancement of sensitivity in plasmonic techniques. We cultured three different types of cells (primary hippocampal neurons, neuroblastoma SH-SY5Y cells, and human embryonic kidney 293 (HEK293) cells) on the grating surface. By introducing KCl solution as a chemical stimulus, we can differentiate the neural activity of distinct cell types and observe the signaling event in a label-free, optical recording platform. This method has potential applications in recording neural signal activity without labeling and stimulation artifacts.
尽管存在各种神经记录和映射技术，但仍有新的技术有待出现。当前的神经成像和记录技术存在侵入性、耗时的标记过程、较差的空间/时间分辨率和嘈杂的信号等问题。神经等离子体学是一种无标记和无毒的记录技术，不存在光漂白或信号平均化的问题。我们引入了一种集成的等离子体-椭圆术平台，用于通过从商业 DVD 中提取的具有成本效益和高质量的光栅来检测膜活性。通过椭圆术技术，人们可以同时测量反射光的幅度（强度）和相位差，同时具有接近表面等离子体共振的高信噪比，这导致等离子体技术的灵敏度得到增强。我们在光栅表面培养了三种不同类型的细胞（原代海马神经元、神经母细胞瘤 SH-SY5Y 细胞和人胚肾 293（HEK293）细胞）。通过引入 KCl 溶液作为化学刺激，我们可以区分不同细胞类型的神经活动，并在无标记和光学记录平台上观察信号事件。该方法在记录神经信号活动而无需标记和刺激伪影方面具有潜在应用。
