Asadishad Tannaz, Sohrabi Foozieh, Hakimi Maryam, Ghazimoradi Mohammad Hossein, Mahinroosta Tayebeh, Hamidi Seyedeh Mehri, Farivar Shirin
Magneto-plasmonic Lab, Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran.
Life Science and Biotechnology Faculty, Shahid Beheshti University, Tehran, Iran.
J Lasers Med Sci. 2021 Aug 26;12:e46. doi: 10.34172/jlms.2021.46. eCollection 2021.
Plasmonic biosensors provide high sensitivity in detecting the low amount of biomarkers and pharmaceutical drugs. We studied the mesenchyme cell activity under the treatment of common sedative drugs of methadone and tramadol using the integrated plasmonic-ellipsometry technique. Mesenchymal stem cells were cultured on patterned plasmonic chips under the treatment of methadone and tramadol drugs. Three cultured chips were kept non-treated as the control ones. The plasmonic-ellipsometry technique was applied to study the signaling characteristic of the cells affected by these two drugs. In this technique, optical information regarding the amplitude ratio and phase change between p- and s-polarized light was recorded. This drug treatment could affect the spectral plasmonic resonance and subsequently the phase shift (Δ) and the amplitude ratio (Ψ) values under p- and s-polarized impinging light. A more significant Δ value for tramadol treatment meant that the phase split was larger between p- and s-polarized light. Tramadol also had more prominent absolute Δ and Ψ values in comparison with methadone. We showed that tramadol caused more contrast in phase shift (Δ) and amplitude ratio (Ψ) between p- and s-polarized impinging light for cultured stem cells in comparison with methadone. It means that tramadol differentiated more the optical responses for p- and s-polarized lights compared to methadone. Our proposed technique possesses the potential of quantitative and qualitative analysis of drugs on humans even on a cell scale.
表面等离子体激元生物传感器在检测低含量生物标志物和药物方面具有高灵敏度。我们使用集成表面等离子体激元 - 椭偏仪技术研究了美沙酮和曲马多这两种常见镇静药物处理下间充质细胞的活性。在美沙酮和曲马多药物处理下，将间充质干细胞培养在图案化的表面等离子体激元芯片上。保留三个未处理的培养芯片作为对照。应用表面等离子体激元 - 椭偏仪技术研究受这两种药物影响的细胞的信号特征。在该技术中，记录了关于p偏振光和s偏振光之间的振幅比和相位变化的光学信息。这种药物处理会影响光谱表面等离子体激元共振，进而影响p偏振光和s偏振光入射下的相移（Δ）和振幅比（Ψ）值。曲马多处理的Δ值更显著，这意味着p偏振光和s偏振光之间的相分离更大。与美沙酮相比，曲马多的绝对Δ和Ψ值也更突出。我们表明，与美沙酮相比，曲马多在培养的干细胞的p偏振光和s偏振光入射之间引起的相移（Δ）和振幅比（Ψ）对比度更大。这意味着与美沙酮相比，曲马多对p偏振光和s偏振光的光学响应区分得更明显。我们提出的技术即使在细胞尺度上也具有对人体药物进行定量和定性分析的潜力。
