Wolfowicz C B, Sakorafas P, Rothstein T L, Marshak-Rothstein A
Department of Microbiology, Boston University Medical Center, Massachusetts 02118.
Clin Immunol Immunopathol. 1988 Mar;46(3):382-95. doi: 10.1016/0090-1229(88)90057-8.
Rheumatoid factors (RF) from individual mice of the MRL/lpr and C3H/lpr strains were examined with regard to target specificity and heavy chain class expression. It was found that MRL/lpr-derived RF preferentially recognize IgG2a target antibodies while C3H/lpr-derived RF exhibit a broader range of cross-reactivity. IgM RF represent only a minor proportion of the RF response in both strains. The IgG2a-specific RF produced by the lpr strains were found to consist of relatively equal proportions of IgG1, IgG2b, IgG3, and IgA antibodies. However, most of the RF produced by a single individual expressed the same heavy chain class and antigen-binding specificity. These results indicate that within an individual lpr mouse, circulating RF are derived from extensive expansion of a limited number of antigen-activated precursors.
对MRL/lpr和C3H/lpr品系的个体小鼠的类风湿因子（RF）进行了靶标特异性和重链类别表达方面的检测。结果发现，源自MRL/lpr的RF优先识别IgG2a靶标抗体，而源自C3H/lpr的RF表现出更广泛的交叉反应性。在这两个品系中，IgM RF仅占RF反应的一小部分。发现lpr品系产生的IgG2a特异性RF由相对等量的IgG1、IgG2b、IgG3和IgA抗体组成。然而，单个个体产生的大多数RF表达相同的重链类别和抗原结合特异性。这些结果表明，在单个lpr小鼠体内，循环RF源自有限数量的抗原激活前体的广泛扩增。
