Warren R W, Sailstad D M, Pisetsky D S
Clin Exp Immunol. 1984 Dec;58(3):731-6.
Monoclonal rheumatoid factors (MoRF) were prepared from autoimmune B6-lpr/lpr mice to investigate the influence of strain background on the specificity of these autoantibodies. Using screening assays for binding to heterologous rabbit IgG, four IgM MoRF were obtained. Three of these antibodies showed a broad pattern of reactivity with IgG antigen, binding BALB/c myeloma IgG1, IgG2a and IgG3 as well as heterologous IgG. One of the antibodies, however, had a distinct form of IgG interaction and was without reactivity with any of the BALB/c myelomas tested. None of the antibodies had significant reactivity with IgG2b. These results suggest common features of B6-lpr/lpr rheumatoid factor (RF) specificities, some of which may be shared by comparable products derived from MRL-lpr/lpr mice. Comparison of these antibodies with those in other reported series suggests that the background genome as well as the nature of the inducing mechanisms may affect the specificity of RF as well as their pathogenetic role.
制备来自自身免疫性B6-lpr/lpr小鼠的单克隆类风湿因子（MoRF），以研究品系背景对这些自身抗体特异性的影响。通过与异源兔IgG结合的筛选试验，获得了四种IgM MoRF。其中三种抗体与IgG抗原呈现广泛的反应模式，能结合BALB/c骨髓瘤IgG1、IgG2a和IgG3以及异源IgG。然而，其中一种抗体具有独特的IgG相互作用形式，与所测试的任何BALB/c骨髓瘤均无反应性。这些抗体均与IgG2b无显著反应性。这些结果表明B6-lpr/lpr类风湿因子（RF）特异性具有共同特征，其中一些特征可能与源自MRL-lpr/lpr小鼠的类似产物相同。将这些抗体与其他报道系列中的抗体进行比较表明，背景基因组以及诱导机制的性质可能会影响RF的特异性及其致病作用。
