Hansen S L, Stewart B J
J Clin Microbiol. 1976 Sep;4(3):227-31. doi: 10.1128/jcm.4.3.227-231.1976.
Two commercially available micromethod multitest systems (API, Analytab Products, Inc., Minitek-Bioquest) were compared with conventional tests suggested by the Center for Disease Control for the identification of anaerobes. Anaerobiosis for the microsystems was achieved using GasPak system (BBL), A total of 175 anaerobes, including 158 clinical isolates and 17 reference strains, were used. Gram morphology, gas-liquid chromatography data, and biochemical reactions from the Center for Disease Control and Virginia Polytechnic Institute anaerobic manuals were used to identify the organisms. The Minitek system included a new anaerobe inoculum broth and two new disks, dextrose without nitrate and nitrate reductase disks. The percentage of correlation of 12 biochemicals using Minitek and 11 biochemicals using the API were compared with the Center for Disease Control reactions. The percentage of correlation of both positive and negative reactions with the API anaerobic strip ranged from 70.8 to 99.4% and with the Minitek from 97.1 to 100%. The microsystems were also evaluated as to the ease of use, adaptabilty to a clinical laboratory, time, and cost.
将两种市售的微量多试验系统（API，Analytab产品公司，Minitek - Bioquest）与疾病控制中心建议的用于厌氧菌鉴定的传统试验进行了比较。使用气体发生袋系统（BBL）实现了微量系统的厌氧环境。总共使用了175株厌氧菌，包括158株临床分离株和17株参考菌株。利用革兰氏形态、气液相色谱数据以及疾病控制中心和弗吉尼亚理工学院厌氧菌手册中的生化反应来鉴定这些微生物。Minitek系统包括一种新的厌氧菌接种肉汤和两种新的圆片，即无硝酸盐葡萄糖圆片和硝酸盐还原酶圆片。将使用Minitek的12种生化反应以及使用API的11种生化反应的相关性百分比与疾病控制中心的反应进行了比较。API厌氧条带的阳性和阴性反应的相关性百分比范围为70.8%至99.4%，而Minitek的为97.1%至100%。还对这些微量系统在易用性、对临床实验室的适应性、时间和成本方面进行了评估。
