Laboratory of Veterinary Dermatology and The Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Republic of Korea.
Laboratory of Veterinary Dermatology and The Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Republic of Korea.
Vet Microbiol. 2021 Feb;253:108957. doi: 10.1016/j.vetmic.2020.108957. Epub 2020 Dec 13.
Staphylococcal cassette chromosome mec (SCCmec) confers methicillin resistance and shows ability for horizontal transfer. However, little is known about the potential transfer of SCCmec between different species of staphylococci in a clinical setting. In this study, we investigated the genetic relationship of SCCmec between staphylococci isolated from dogs affected with pyoderma and their owners. Clinical isolates were collected from pyoderma lesions of dogs and from the nasal cavity and finger of owners. Clonal lineages were characterized using multi-locus sequence typing. Genetic relatedness of SCCmec in the isolates from dogs and owners was first evaluated with dru and SCCmec typing, and whole-genome sequencing (WGS) was used to confirm the similarity of DNA sequences and the structural composition of SCCmec. A total of 100 Staphylococcus strains were isolated from 31 dog-owner pairs. One pair with isolates carrying the same SCCmec type V and dru type 11a was detected: 18D20-1 (S. pseudintermedius, dog), 18D20-2 (S. schleiferi, dog), and 18H20-F2 (S. epidermidis, dog owner). WGS revealed that these three isolates showed remarkable genetic similarity in SCCmec with respect to DNA sequences, dru type, structure composition of ccrC and the mec complex, and DR-1 in orfX, which is considered to be the insertion site of SCCmec. Entire identical nucleotide sequences of the whole SCCmec region in different Staphylococcus strains were absent between dogs and owners. However, the remarkable genetic similarity of SCCmec from staphylococci isolated from a dog and owner pair emphasizes that antimicrobial resistance surveillance adopted One Health concept should be continuously performed.
葡萄球菌盒式染色体 mec（SCCmec）赋予耐甲氧西林的能力，并表现出水平转移的能力。然而，在临床环境中，关于不同种葡萄球菌之间 SCCmec 潜在转移的了解甚少。在本研究中，我们调查了患有脓皮病的犬及其主人分离的葡萄球菌之间 SCCmec 的遗传关系。临床分离株从犬的脓皮病病灶以及主人的鼻腔和手指中采集。使用多位点序列分型来表征克隆谱系。首先使用 dru 和 SCCmec 分型评估来自犬和主人的分离物中 SCCmec 的遗传相关性，然后使用全基因组测序（WGS）来确认 DNA 序列的相似性和 SCCmec 的结构组成。从 31 对犬-主人中分离出 100 株葡萄球菌。检测到一对携带相同 SCCmec 类型 V 和 dru 类型 11a 的分离株：18D20-1（中间葡萄球菌，犬），18D20-2（施氏葡萄球菌，犬）和 18H20-F2（表皮葡萄球菌，犬主人）。WGS 表明，这三个分离物在 SCCmec 方面的 DNA 序列、dru 类型、ccrC 和 mec 复合物的结构组成以及 orfX 中的 DR-1 方面表现出显著的遗传相似性，后者被认为是 SCCmec 的插入位点。在犬和主人之间，不同葡萄球菌菌株的整个 SCCmec 区域的整个核苷酸序列并不完全相同。然而，来自犬和主人分离物的 SCCmec 的显著遗传相似性强调了应该持续进行采用 One Health 概念的抗生素耐药性监测。
