Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.
Plant and Microbial Research Unit, Division of Plant and Microbial Sciences, Institute of Agrobiological Sciences, National Agriculture and Food Research Organization, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan.
Arch Virol. 2021 Feb;166(2):645-649. doi: 10.1007/s00705-020-04916-9. Epub 2021 Jan 1.
Pleioblastus mosaic virus (PleMV) is a tentative member of the genus Potyvirus in the family Potyviridae and was discovered in bamboo with mosaic symptoms in Tokyo, Japan. Since no information on the genome sequence of PleMV has been reported, its taxonomic position has long been uncertain. Here, we report the first complete genome sequences of two distinct PleMV isolates. Excluding the 3'-terminal poly(A) tail, their genomic RNA sequences consist of 9,634 and 9,643 nucleotides (nt); both contain a large open reading frame (ORF) encoding a polyprotein and a small ORF termed PIPO. The large ORFs of the two isolates share 79.2% and 87.6% sequence identity at the nucleotide (nt) and amino acid (aa) level, respectively, and were found to have the highest nt and aa sequence identity (69.0% and 69.9%) to the potyvirus johnsongrass mosaic virus (JGMV). Phylogenetic analysis showed that PleMV is most closely related to JGMV but forms its own clade. These results suggest that PleMV is a distinct member of the genus Potyvirus.
重叶律草花叶病毒（Pleioblastus mosaic virus，PleMV）是马铃薯 Y 病毒科马铃薯 Y 属的暂定成员，于日本东京的出现花叶症状的竹子中发现。由于没有关于 PleMV 基因组序列的信息，其分类地位一直不确定。在这里，我们报告了两个不同 PleMV 分离株的第一个完整基因组序列。不包括 3'-末端聚（A）尾，其基因组 RNA 序列由 9634 和 9643 个核苷酸（nt）组成；两者均包含一个编码多聚蛋白的大开放阅读框（ORF）和一个称为 PIPO 的小 ORF。两个分离株的大 ORF 在核苷酸（nt）和氨基酸（aa）水平上分别具有 79.2%和 87.6%的序列同一性，并且与马铃薯 Y 病毒属的约翰逊草花叶病毒（johnsongrass mosaic virus，JGMV）具有最高的 nt 和 aa 序列同一性（69.0%和 69.9%）。系统发育分析表明，PleMV 与 JGMV 最密切相关，但形成自己的分支。这些结果表明 PleMV 是马铃薯 Y 病毒属的一个独特成员。
