College of Landscape and Horticulture, Yunnan Agricultural University, Kunming, 650201, China.
Library, Yunnan Agricultural University, Kunming, 650201, China.
Arch Virol. 2020 Aug;165(8):1915-1918. doi: 10.1007/s00705-020-04663-x. Epub 2020 Jun 5.
We determined the complete genomic sequence of begonia flower breaking virus (BFBV), a novel putative member of the genus Potyvirus isolated from Begonia bowerae cv. 'Tiger' plants grown in Kunming. The genomic RNA comprises 9540 nucleotides (nt), excluding the 3'-terminal poly(A) tail, and contains a typical open reading frame (ORF) of potyviruses. The ORF consists of 9219 nucleotides and encodes a 3073-amino-acid polyprotein that is predicted to be proteolytically cleaved into 10 mature peptides. Sequence comparison indicated that BFBV shares 43.9-55.12% amino acid sequence identity with known potyviruses and that BFBV shares the highest amino acid sequence identity (55.12%) with beet mosaic virus. The results from the complete genomic sequence analysis further suggest that BFBV is a member of a novel species in the genus Potyvirus.
我们测定了从昆明种植的四季秋海棠 '老虎'品种中分离得到的新型暂定番茄丛矮病毒（BFBV）的全基因组序列。该基因组 RNA 由 9540 个核苷酸（nt）组成，不包括 3'-末端 poly(A)尾，含有典型的马铃薯 Y 病毒属（Potyvirus）的开放阅读框（ORF）。ORF 由 9219 个核苷酸组成，编码一个 3073 个氨基酸的多聚蛋白，预计该多聚蛋白经蛋白水解后可裂解为 10 个成熟肽。序列比较表明，BFBV 与已知的马铃薯 Y 病毒属病毒的氨基酸序列同一性为 43.9-55.12%，与甜菜曲顶病毒的氨基酸序列同一性最高（55.12%）。完整基因组序列分析的结果进一步表明，BFBV 是马铃薯 Y 病毒属中的一个新种成员。
