• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

与 AAO 家族基因相比，GhAAO2 被观察到在棉花中对 NaHCO 胁迫有反应。

GhAAO2 was observed responding to NaHCO stress in cotton compared to AAO family genes.

机构信息

Institute of Cotton Research of Chinese Academy of Agricultural Sciences / Zhengzhou Research Base, State Key Laboratory of Cotton Biology, School of Agricultural Sciences, Zhengzhou University, Anyang, 455000, Henan, China.

Engineering Research Centre of Cotton, Ministry of Education / College of Agriculture, Xinjiang Agricultural University, 311 Nongda East Road, Urumqi, 830052, China.

出版信息

BMC Plant Biol. 2022 Dec 20;22(1):603. doi: 10.1186/s12870-022-03999-7.

DOI:10.1186/s12870-022-03999-7
PMID:36539701
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9768942/
Abstract

BACKGROUND

Abscisic acid (ABA) is an important stress hormone, the changes of abscisic acid content can alter plant tolerance to stress, abscisic acid is crucial for studying plant responses to abiotic stress. The abscisic acid aldehyde oxidase (AAO) plays a vital role in the final step in the synthesis of abscisic acid, therefore, understanding the function of AAO gene family is of great significance for plants to response to abiotic stresses.

RESULT

In this study, 6, 8, 4 and 4 AAO genes were identified in four cotton species. According to the structural characteristics of genes and the traits of phylogenetic tree, we divided the AAO gene family into 4 clades. Gene structure analysis showed that the AAO gene family was relatively conservative. The analysis of cis-elements showed that most AAO genes contained cis-elements related to light response and plant hormones. Tissue specificity analysis under NaHCO stress showed that GhAAO2 gene was differentially expressed in both roots and leaves. After GhAAO2 gene silencing, the degree of wilting of seedlings was lighter than that of the control group, indicating that GhAAO2 could respond to NaHCO stress.

CONCLUSIONS

In this study, the AAO gene family was analyzed by bioinformatics, the response of GhAAO gene to various abiotic stresses was preliminarily verified, and the function of the specifically expressed gene GhAAO2 was further verified. These findings provide valuable information for the study of potential candidate genes related to plant growth and stress.

摘要

背景

脱落酸（ABA）是一种重要的应激激素，其含量的变化可以改变植物对胁迫的耐受性，ABA 对于研究植物对非生物胁迫的响应至关重要。脱落酸醛氧化酶（AAO）在 ABA 合成的最后一步中起着至关重要的作用，因此，了解 AAO 基因家族的功能对于植物应对非生物胁迫具有重要意义。

结果

在这项研究中，在四个棉花物种中分别鉴定出 6、8、4 和 4 个 AAO 基因。根据基因的结构特征和系统发育树的特征，我们将 AAO 基因家族分为 4 个分支。基因结构分析表明，AAO 基因家族相对保守。顺式元件分析表明，大多数 AAO 基因含有与光反应和植物激素相关的顺式元件。在 NaHCO 胁迫下的组织特异性分析表明，GhAAO2 基因在根和叶中均有差异表达。沉默 GhAAO2 基因后，幼苗的萎蔫程度比对照组轻，表明 GhAAO2 可以响应 NaHCO 胁迫。

结论

本研究通过生物信息学对 AAO 基因家族进行了分析，初步验证了 GhAAO 基因对各种非生物胁迫的响应，并进一步验证了特异性表达基因 GhAAO2 的功能。这些发现为研究与植物生长和胁迫相关的潜在候选基因提供了有价值的信息。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/4fc8ab690824/12870_2022_3999_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/730e87c12e2e/12870_2022_3999_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/e9e864465afa/12870_2022_3999_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/cf952da289ee/12870_2022_3999_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/57ac2b3a2c39/12870_2022_3999_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/c2600dcb1e29/12870_2022_3999_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/e07190516684/12870_2022_3999_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/d5977074a6cb/12870_2022_3999_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/ee8178f96f68/12870_2022_3999_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/25505c0a36db/12870_2022_3999_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/8ee7aaa39500/12870_2022_3999_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/89c6fc6c2cb5/12870_2022_3999_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/4fc8ab690824/12870_2022_3999_Fig12_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/730e87c12e2e/12870_2022_3999_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/e9e864465afa/12870_2022_3999_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/cf952da289ee/12870_2022_3999_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/57ac2b3a2c39/12870_2022_3999_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/c2600dcb1e29/12870_2022_3999_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/e07190516684/12870_2022_3999_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/d5977074a6cb/12870_2022_3999_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/ee8178f96f68/12870_2022_3999_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/25505c0a36db/12870_2022_3999_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/8ee7aaa39500/12870_2022_3999_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/89c6fc6c2cb5/12870_2022_3999_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/34b9/9768942/4fc8ab690824/12870_2022_3999_Fig12_HTML.jpg

相似文献

1
GhAAO2 was observed responding to NaHCO stress in cotton compared to AAO family genes.与 AAO 家族基因相比，GhAAO2 被观察到在棉花中对 NaHCO 胁迫有反应。
BMC Plant Biol. 2022 Dec 20;22(1):603. doi: 10.1186/s12870-022-03999-7.
2
Molecular structures and functional exploration of NDA family genes respond tolerant to alkaline stress in Gossypium hirsutum L.棉花 NDA 家族基因的分子结构和功能探索对碱性胁迫的耐受性。
Biol Res. 2022 Jan 21;55(1):4. doi: 10.1186/s40659-022-00372-8.
3
GhIMP10D, an inositol monophosphates family gene, enhances ascorbic acid and antioxidant enzyme activities to confer alkaline tolerance in Gossypium hirsutum L.GhIMP10D，一种肌醇单磷酸家族基因，通过提高抗坏血酸和抗氧化酶活性赋予棉花的耐碱性。
BMC Plant Biol. 2023 Sep 22;23(1):447. doi: 10.1186/s12870-023-04462-x.
4
Characterization and Identification of Drought-Responsive ABA-Aldehyde Oxidase (AAO) Genes in Potato ( L.).马铃薯（L.）中干旱响应型脱落酸醛氧化酶（AAO）基因的表征与鉴定
Plants (Basel). 2023 Nov 9;12(22):3809. doi: 10.3390/plants12223809.
5
Response of phytohormone mediated plant homeodomain (PHD) family to abiotic stress in upland cotton (Gossypium hirsutum spp.).植物激素介导的植物同源异型结构域（PHD）家族对陆地棉（陆地棉种）非生物胁迫的响应。
BMC Plant Biol. 2021 Jan 6;21(1):13. doi: 10.1186/s12870-020-02787-5.
6
Histone Deacetylase (HDAC) Gene Family in Allotetraploid Cotton and Its Diploid Progenitors: In Silico Identification, Molecular Characterization, and Gene Expression Analysis under Multiple Abiotic Stresses, DNA Damage and Phytohormone Treatments.组蛋白去乙酰化酶（HDAC）基因家族在异源四倍体棉花及其二倍体祖先中的鉴定：在多种非生物胁迫、DNA 损伤和植物激素处理下的计算机识别、分子特征和基因表达分析。
Int J Mol Sci. 2020 Jan 3;21(1):321. doi: 10.3390/ijms21010321.
7
Genome-wide identification and expression analysis of Raffinose synthetase family in cotton.棉花中棉子糖合成酶家族的全基因组鉴定与表达分析
BMC Bioinformatics. 2021 Jun 29;22(1):356. doi: 10.1186/s12859-021-04276-4.
8
Evolution of AITR family genes in cotton and their functions in abiotic stress tolerance.棉花 AITR 家族基因的进化及其在非生物胁迫耐受性中的功能。
Plant Biol (Stuttg). 2021 May;23 Suppl 1:58-68. doi: 10.1111/plb.13218. Epub 2021 Jan 26.
9
Sequence Characteristics and Expression Analysis of Gene in Upland Cotton ( L.).陆地棉基因序列特征与表达分析。
Int J Mol Sci. 2022 Oct 10;23(19):12040. doi: 10.3390/ijms231912040.
10
The PIN gene family in cotton (Gossypium hirsutum): genome-wide identification and gene expression analyses during root development and abiotic stress responses.棉花（陆地棉）中的PIN基因家族：全基因组鉴定以及根系发育和非生物胁迫响应过程中的基因表达分析
BMC Genomics. 2017 Jul 3;18(1):507. doi: 10.1186/s12864-017-3901-5.

引用本文的文献

1
Plant immunity to insect herbivores: mechanisms, interactions, and innovations for sustainable pest management.植物对昆虫食草动物的免疫：可持续害虫管理的机制、相互作用及创新
Front Plant Sci. 2025 Jul 22;16:1599450. doi: 10.3389/fpls.2025.1599450. eCollection 2025.
2
Characterization of Cd and As accumulation and subcellular distribution in different varieties of perennial ryegrasses.不同品种多年生黑麦草中镉和砷的积累及亚细胞分布特征
BMC Plant Biol. 2025 Apr 22;25(1):508. doi: 10.1186/s12870-025-06530-w.
3
Raffinose degradation-related gene was screened out responding to salinity stress through expression patterns of family genes.

本文引用的文献

1
Genome-wide expression analysis of phospholipase A1 (PLA1) gene family suggests phospholipase A1-32 gene responding to abiotic stresses in cotton.对磷脂酶 A1（PLA1）基因家族的全基因组表达分析表明，磷脂酶 A1-32 基因在棉花中对非生物胁迫有反应。
Int J Biol Macromol. 2021 Dec 1;192:1058-1074. doi: 10.1016/j.ijbiomac.2021.10.038. Epub 2021 Oct 14.
2
Genome-wide identification of CK gene family suggests functional expression pattern against Cd stress in Gossypium hirsutum L.全基因组鉴定棉花 CK 基因家族对 Cd 胁迫的功能表达模式
Int J Biol Macromol. 2021 Oct 1;188:272-282. doi: 10.1016/j.ijbiomac.2021.07.190. Epub 2021 Aug 5.
3
通过家族基因的表达模式筛选出响应盐胁迫的棉子糖降解相关基因。
Front Plant Sci. 2023 Dec 19;14:1246677. doi: 10.3389/fpls.2023.1246677. eCollection 2023.
4
Characterization and Identification of Drought-Responsive ABA-Aldehyde Oxidase (AAO) Genes in Potato ( L.).马铃薯（L.）中干旱响应型脱落酸醛氧化酶（AAO）基因的表征与鉴定
Plants (Basel). 2023 Nov 9;12(22):3809. doi: 10.3390/plants12223809.
Cotton transcriptome analysis reveals novel biological pathways that eliminate reactive oxygen species (ROS) under sodium bicarbonate (NaHCO) alkaline stress.
棉花转录组分析揭示了在碳酸氢钠（NaHCO₃）碱性胁迫下消除活性氧（ROS）的新生物学途径。
Genomics. 2021 May;113(3):1157-1169. doi: 10.1016/j.ygeno.2021.02.022. Epub 2021 Mar 6.
4
Complete genome sequence of pleioblastus mosaic virus, a distinct member of the genus Potyvirus.丛生竹丛簇矮化病毒全基因组序列：一个 distinct member of the genus Potyvirus（马铃薯 Y 属的一个独特成员）。
Arch Virol. 2021 Feb;166(2):645-649. doi: 10.1007/s00705-020-04916-9. Epub 2021 Jan 1.
5
TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data.TBtools：一个用于生物大数据交互式分析的集成工具包。
Mol Plant. 2020 Aug 3;13(8):1194-1202. doi: 10.1016/j.molp.2020.06.009. Epub 2020 Jun 23.
6
Genome-wide expression analysis suggests glutaredoxin genes response to various stresses in cotton.全基因组表达分析表明谷氧还蛋白基因对棉花各种胁迫的响应。
Int J Biol Macromol. 2020 Jun 15;153:470-491. doi: 10.1016/j.ijbiomac.2020.03.021. Epub 2020 Mar 5.
7
Genome-wide identification and expression analysis of PUB genes in cotton.棉花 PUB 基因的全基因组鉴定和表达分析。
BMC Genomics. 2020 Mar 6;21(1):213. doi: 10.1186/s12864-020-6638-5.
8
Differentially expressed bZIP transcription factors confer multi-tolerances in Gossypium hirsutum L.差异表达的 bZIP 转录因子赋予棉花多耐性。
Int J Biol Macromol. 2020 Mar 1;146:569-578. doi: 10.1016/j.ijbiomac.2020.01.013. Epub 2020 Jan 7.
9
Gossypium barbadense and Gossypium hirsutum genomes provide insights into the origin and evolution of allotetraploid cotton.海岛棉和陆地棉基因组为研究异源四倍体棉的起源和进化提供了线索。
Nat Genet. 2019 Apr;51(4):739-748. doi: 10.1038/s41588-019-0371-5. Epub 2019 Mar 18.
10
Salinity and crop yield.盐度和作物产量。
Plant Biol (Stuttg). 2019 Jan;21 Suppl 1:31-38. doi: 10.1111/plb.12884. Epub 2018 Sep 5.