Department of Biotechnology, Thapar Institute of Engineering and Technology, Patiala, Punjab, 147004, India.
World J Microbiol Biotechnol. 2024 Jul 20;40(9):274. doi: 10.1007/s11274-024-04083-y.
Argemone mexicana belonging to family Papaveraceae is a traditional medicinal plant widely utilized by tribal people in India for treating various ailments like skin infections, wounds and inflammation. This plant is very rich in alkaloidal content, which has a great potential in the treatment of anti-inflammatory disorders. Therapeutically promising bioactive molecules are often produced by endophytic fungi associated with medicinal plants. In this investigation, endophytic fungi were isolated from various parts of A. mexicana and screened for alkaloidal content. Among these, one of the fungal isolate, Acremonium alternatum AMEF-5 producing maximum alkaloids showed significant anti-inflammatory activity. Fractionation of this crude fungal extract through column chromatography yielded eight fractions, which were further screened for anti-inflammatory activities. Fraction 3 exhibited significant anti-inflammatory activity by the inhibition of lipoxygenase enzyme (IC 15.2 ± 0.09 µg/ml), scavenging of the nitric oxide radicals (IC 11.38 ± 0.35 µg/ml), protein denaturation (IC 14.93 ± 0.4 µg/ml), trypsin inhibition (IC 12.06 ± 0.64 µg/ml) and HRBC stabilization (IC 11.9 ± 0.22 µg/ml). The bioactive alkaloid in fraction 3 was identified as aconitine which was confirmed by UV, FTIR, HPLC, HRMS, H NMR, and C NMR analysis. This study demonstrates that endophytic fungi serve a potential source for sustainable production of therapeutically important alkaloids.
墨西哥天芥菜属于罂粟科，是一种传统药用植物，在印度被部落人民广泛用于治疗各种疾病，如皮肤感染、伤口和炎症。这种植物含有丰富的生物碱，具有治疗抗炎紊乱的巨大潜力。与药用植物相关的内生真菌通常会产生具有治疗潜力的生物活性分子。在这项研究中，从墨西哥天芥菜的不同部位分离出内生真菌，并对其生物碱含量进行筛选。在这些真菌中，一种产生最多生物碱的真菌分离株——交替镰刀菌 AMEF-5 表现出显著的抗炎活性。通过柱层析对该粗真菌提取物进行分级分离，得到 8 个馏分，进一步筛选其抗炎活性。馏分 3 通过抑制脂氧合酶酶（IC 15.2 ± 0.09 µg/ml）、清除一氧化氮自由基（IC 11.38 ± 0.35 µg/ml）、蛋白质变性（IC 14.93 ± 0.4 µg/ml）、胰蛋白酶抑制（IC 12.06 ± 0.64 µg/ml）和 HRBC 稳定（IC 11.9 ± 0.22 µg/ml），表现出显著的抗炎活性。馏分 3 中的生物活性生物碱被鉴定为乌头碱，这通过 UV、FTIR、HPLC、HRMS、H NMR 和 C NMR 分析得到了证实。这项研究表明，内生真菌是可持续生产治疗上重要生物碱的潜在来源。
